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Why is a needle used to isolate individual colonies from a spread plate or streak plate?
Blondedummie2
Between each set of streaks you sterilize the inoculating loop in the Bunsen flame. At the beginning of the next streak you overlap with the end of the one before. The effect of the technique is to "dilute" the bacteria by gradually spreading then over greater distances. Eventually, a point is reached where single bacteria are spaced sufficiently far apart for single colonies to grow without infringing their neighbors.
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∙ 8y ago
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∙ 14y agoI believe it is necessary to isolate individual colonies from a mixed broth growth because it is the first step in achieving a pure culture for further studies and testing.
Wiki User
∙ 12y agoIt is much smaller and easier to sterilize during transport. Much more effective instrument.
Wiki User
∙ 8y agoYou need to isolate bacterial colonies in order to obtain pure cultures of a particular specie.
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∙ 9y agoIt is important to isolate bacterial colonies because bacteria is omnipresent and can thrive in communities of mixed population.
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∙ 10y agowhich structure can be found in both eukaryotic and prokaryoutic cells
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Suppose you have two organisms in a mixture and organism A is 1000 times more abundant than organism B Will you be able to isolate organism B using the spread plate technique?
yes it can becuz it can be able to isolate on different plates
What are the Different methods used to isolate microorganisms?
1 The Spread Plate: If a mixture of cells is spread out on an agar surface so that every cell grows into a completely separate colony, a macroscopically visible growth or cluster of microorganisms on a solid medium, each colony represents a pure culture. The spread plate is an easy, direct way of achieving this 2 The Pour Plate: Extensively used with bacteria and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating result. 3 The streak plate: Pure colonies also can be obtained from streak plates. The microbial mixture is transferred to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in several patterns
Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.
What area of a streak plate will contain the greatest amount of growth?
The areas that are streaked by the inoculating loop will have greater growth than the areas that were not streaked. This is because at some point on the streaks the individual cells will have been removed from the loop as the loop glides along the agar surface and will give rise to separate colonies. As the colonies grow they will spread to other areas that are not contaminated in the agar. This is an easy answer and don't know why someone has not put and answer to it. ---------------------------------------------------------------------------- - I guess the area that this question is talking about is the quadrants in which the streak are placed. therefore the area with greatest amount will be the 1 quadrant (1st streak) because it has the most conc amount of microorganism .. then the 4th quadrant(last streak) has the least amount of growth and it's main purpose is to isolate the microrganism :) - anonymous :DD
1 how are microorganisms diluted and spread out to form individual colonies In the streak plate technique?
Bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and then streaked out in one of several patterns. At some point, individual cells will be removed from the loop and will give rise to separate colonies.source: http://quizlet.com/17578430/micro-lab-unit-1ex-15-16-streak-plate-technique-flash-cards/
What field of biology is observing colonies of bacteria?
microbiology normal the bacteria are grown on agar plates atsrting out with a dense area and spread out to in the end isolate individual colonies and have some hope of identifying them
Why a needle drowned in water and a ship does not?
because a ship has a wider area to spread it`s surface on and a needle does not because a ship has a wider area to spread it`s surface on and a needle does not
Can streak or spread plates isolate pure cultures from mixed bacteria?
YES
How was disease spread in the colonies?
mosquitoes
Can leukemia be spread through a used needle and what would the cell life be on the used needle?
For male sometimes same for females
How did American colonist spread news?
how did the colonists spread news to other colonies about what was happening
How did war in Europe spread to the American colonies?
They spread by people trying to kill each other.
How the wars in Europe spread to the American colonies?
They spread by people trying to kill each other.
What is involved in a needle aspiration biopsy?
A needle is used to withdraw fluid from lymph nodes located near the growth, to make sure the cancer has not spread to these nodes.
What was the geographic location of the first great awakening?
Started in the New England colonies and spread to the rest of the colonies.
What was William h Seward's goal?
spread American ideas of democracy and individual freedom.
Who spread the news of The Intolerable Acts to the colonies?
Committees of Correspondence
