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Coagulase is a protein enzyme. It is important to use fresh cultures in a coagulase test because coagulase expression is highest during the log phase of growth.
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What are the biochemical tests to confirm staphylococcus?
Streptococci are not differentiated by biochemical tests, but rather by hemolysis reaction (beta Strep or alpha Strep) and sero-group (as in Group E Strep).Further, streptococcus is also identified by thr following tests:1. Coagulase.2. Haemolysin (It lyses 4% RBC suspension)3. Fibrinolysin (It can dissolve a fibrin clot)4. Strp. Pneumoniae also gives a positive bile solubility test.5. It ferments Inulin (A sugar)
Did you get a pure culture in your subculture and how to know?
Ensuring that your culture is pure is very important to getting accurate results of your gram stain. One way to tell if your culture is pure is to test both the control and the experiment cultures. If you get a different result than the one you were expecting, you might have a corrupted culture. Making sure that you have several items of each is helpful, as well.
Why must fresh bacterial cultures be used in a Gram stain?
Why must young cultures be used when doing a Gram stain Young cultures must be used so the crystal violet can stick to the cell walls of Gram positive bacteria. The cell walls break down in old cultures and the staining process is not accurate
Why is CBC so important?
CBC test importance its basic test ,blood composition ,complications in the shape of cells,and esr ,this test show other assessment of diseases
What cell part fills most of the cell?
The CytoPlasm trust me its correct no need to panic especially if u r doing an important test or Exam
What is difference between tube coagulase and slide coagulase test?
tube coagulase is done in test tube while slide coagulase is done on slide.thnk you all! cheeres!
Is coagulase test a microbiological test?
YES
What is a coagulase test used for?
Rabbit plasma, sometimes with EDTA added
Test used for the identification of Staphylococcus aureus?
staph. aureus test positive for catalase test and coagulase test consecutively
What is a free coagulase and its significance?
Free coagulase is an extracellular toxin that reacts with a thrombin-like, thermostable substance known as coagulase-reacting factor (CRF) or serum factor. The reaction of coagulase and CRF will lead to the formation of coagulase-CRF complex where this complex indirectly changes frbrinogen to fibrin (clotting factor) and hence will form a fibrin clot. Furthermore, it is also use as the primary indicator of virulence among staphylococci. Its clinical significance is that when doing the coagulase tube test, the free coagulase will react with the plasma in the tube and hence convert the fribrinogen into fibrin which lead to fibrin clot formation in the tube. This is shown after one day of incubation of the tube, when the tube being invert, the plasma will not go down showing that it is clotted.
What is the principle behind the coagulase test?
in the coagulase test, normally a virulent staphylococcus is put into plasma. plasma contains the plasma protein fibrinogen. when fibrinogen is acted upon by a fibrinogenase, it is then converted to fibrin. fibrin has te ability to form clots. so in the case of staphylococcus aureus which has the enzyme coagulase also called fibrinogenase, the enzyme acts on the fibrinogen in plasma which is converted to fibrin hence the clots that are seen suspending in the solution after 4 hours.
What are the general characteristics of staphylococcus Aureus?
Gram + coccus. Positive methanol test. Yellow on agar.
Is citrate test results for Staphylococcus aureus?
No, Catalase +ve, Coagulase +ve, Gram + cocci. Citrate test is usually used to differentiate members of the Family Enterobacteriaceae. Check Bergeys systematic bacteriology. 1980
What color test tube is for blood cultures?
The yellow evacuated tubes are for blood cultures.
What bacterial product does staphaurex test for?
Staphaurex is a rapid slide agglutination procedure for differentiating Staphylococci that possess coagulase and/or protein A. Particularly Staphylococcus aureus, from Staphylococci that possess neither of these factors.
How do you test the viability of microbial cultures?
Viability of microbial cultures can be tested by doing "Vital Staining"
What are the biochemical tests to confirm staphylococcus?
Streptococci are not differentiated by biochemical tests, but rather by hemolysis reaction (beta Strep or alpha Strep) and sero-group (as in Group E Strep).Further, streptococcus is also identified by thr following tests:1. Coagulase.2. Haemolysin (It lyses 4% RBC suspension)3. Fibrinolysin (It can dissolve a fibrin clot)4. Strp. Pneumoniae also gives a positive bile solubility test.5. It ferments Inulin (A sugar)
