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It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.

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Kyla Klocko

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2y ago
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Demetris Boehm

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2y ago

to have an isolatedcolonies

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7y ago

When you do this step, you are making sure that the loop is cool enough to put into the broth. Since both the loop and the agar are sterile, you are not going to contaminate the loop. Waving it the air or holding in the air for a long time can pick up bacteria or other microbes floating in the air.

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13y ago

to have an isolatedcolonies

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Q: Why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
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When getting inoculums from a slant why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?

It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.


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What actions with sterile gowns and gloves violate correct sterile technique?

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