It allows one to determine the amoun of oxygen present by observing the color of the resazurin.
it is a type of media used for the growth of obligate anaerobes.
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.
Blood agar is media rich in reb blood cells and the most commonly used type of media is horse blood media. It is useful for the isolation of bacteria such as Streptococci or organisms that would not readily grow on media that lack red blood cells. For the isolation of Streptococci it is also useful in observing alpha and beta haemolysis.
The use of a medium that is both selective and differential can be justified in a few ways. They allowe the growth of organisms that can be of interest. The media can also be quite useful diagnostically in medical settings.
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Resazurin dye is used in microbiological culture media for anaerobes as an indicator of the presence of oxygen. The dye turns pink when oxygen is present.
it is a type of media used for the growth of obligate anaerobes.
Anaerobes cannot be cultivated in the presence of atmospheric oxygen.
If its Fluid thioglycollate media - a red colour indicates thatg the media has been oxygenated - which is not desirable. The colour change is due to a chemical called resazurin in the media.
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they are those organisms that are used to grow anaerobes so they can decreaes the amount of oxgyen present
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To culture obligate anaerobes, all molecular oxygen must be removed and kept out of the medium. Addition of oxygen-binding agents such as thioglycollate, the amino acid cysteine, or sodium sulfide to the medium prevents oxygen from killing the anaerobes you want to culture.
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The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.