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Because the osmotic pressure of "plain" water can be too much for bacteria, causing them to pop, and throw off your colony counts during your serial dilutions. A buffered saline solution keeps the bacteria at their usual osmotic pressure. Typical saline is 0.85%.
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Why is saline used in microbiology?
Saline is sterilized and used to suspend and wash bacterial cells. Saline is used as distilled water would cause the cells to lyse due to osmosis. The saline solution is a solution of salt and water, it contains no source of energy.
PH of physiological saline?
No specific pH
Why saline solution is used in this silt test?
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What medicine use in bacterial infection?
anti-biotics are used in an bacterial infection. If it is serious they will put you on a drip.
What is the process of adding foreign DNA to a bacterial cell called?
The process of adding foreign DNA to a bacterial cell is called Bacterial Transformation. It is a technique used very frequently in molecular Biology labs.Ê
Why is saline used in serial dilution?
Because the osmotic pressure of "plain" water can be too much for bacteria, causing them to pop, and throw off your colony counts during your serial dilutions. A buffered saline solution keeps the bacteria at their usual osmotic pressure. Typical saline is 0.85%.
Why is saline used for serial dilution?
Ringers solution contains a salt content similar to what is found in the cells of a bacteria. This prevents the bacteria under going osmotic stress and subsequent bursting of the cell walls.
Why is saline used in microbiology?
Saline is sterilized and used to suspend and wash bacterial cells. Saline is used as distilled water would cause the cells to lyse due to osmosis. The saline solution is a solution of salt and water, it contains no source of energy.
What is a ten fold serial dilution?
Serial dilution is usually 1/10 dilution. Therefore after a series of dilutions, you have a logarithmic curve of concentration (log10). Basically, if diluting 1/10 and starting off with 1 molar solution, first dilution = 0.1M, 2nd = 0.01M, 3rd = 0.001M. If making a 0.001M solution involved weighing out 0.005g of a salt for example, the error in making this solution out would be very large in comparison to weighing out 5g (1M) and diluting it 3 times by serial dilution. The benefit of it is mainly accuracy.
What test is used to further identify unknown gram negative?
Serial dilution technique
Is Saline NaCl?
Not exactly. Saline solution is sterilized and is a dilution of sodium chloride (NaCl) - meaning that the NaCl is dissolved in deionized water. The solution is 9 grams of sodium chloride (NaCl) dissolved in 1 liter of water.
Can sterile saline be used interchangeably with injectable saline?
no sterile saline cannot be used because strile saline is different from injectable saline. strile saline is used for irrigating the wound and injectable saline in given intravenously
Why is NSS or 0.85 percent NaCl solution used in emulsifying the bacterial smear?
0.85% nacl sol or normal saline solution is used as it is an isotonic solution which will emmulsify bacterial cells and dont cause any injury ( injury due to osmotic gradient in which cell can shrink or swell/burst ). solution upto 0.98% can be used.
In the use-dilution test a chemical is evaluated by its ability to kill 106 and 108 dried clostridium sporongenes or bacillus subtilis endospores. why is this considered a stringent test?
Chemicals do not readily penetrate bacterial endospores. The number of endospores used is higher than would be expected in normal use of disinfectant. It uses a measure of determining the effectiveness of a disinfectant using serial dilutions.
What unit of measurement is used for bacterial colonies?
Many aspects of bacterial colonies may be measured for example colony size or appearance can be useful. The measurement which is perhaps the most useful however, is the colony forming units per ml (or cfu) which indicates how many bacterial cells are present to form colonies on the medium. This is a relatively simple calculation which is done by initially counting the number of colonies present on the medium. this number is then multiplied by the inverse of the dilution used. For example, if the dilution of the bacterial broth was 10-2 and the number of colonies on the medium was 60, then the calculation would be 60 x 102. This result is then divided by the volume of sample used in ml and will give you the number of colony forming units per ml present on the medium. Ben McD
Is dilution an acceptable way to dispose of the used copper chloride solution?
Is dilution an acceptable way to dispose of the used copper chloride solution
What is the formula used to calculate dilution ratios?
A dilution ratio is normally used for a mixture of two fluids: an active component and a carrier solvent. The dilution ratio is the ratio of the volume of the solvent to the volume of the active component.
