Microorganisms in the hay infusion are not stained because the microorganisms are gram negative and if we are trying to use stain them, only gram positive organisms can pick up.
because they are gram negative
Hay infusion consists of microorganisms like protozoa. Most of the protozoa are motile in nature since they have locomotive structures like flagella, cilia and pseudopods that's why the organisms that can be found in hay infusion generally moves.
Hay infusion is a method of making a suitable home for your microorganisms to grow, this is achieved by boiling hay with water. This results to a broth, rich with carbohydrates and other "food" for your microorganisms extracted from the hay you used. Now, to make your microorganisms grow in numbers in that infusion, you need to get a little amount of the sample that you want to test for the presence of microorganisms. Example, getting a few milliliters of pond water, then pouring it to your hay-infusion. We will leave this for 24-48 hours so that the microorganisms will be able to multiply and grow in your infusion. Now, regarding the question on prokaryotic and eukaryotic cells... These two are actually the types of cells animals may have. Prokaryotic cells are said to be primitive and are commonly seen in bacteria, while eukaryotic cells are more advanced cells seen in higher forms of life like us. However, in the infusion you prepared, after pouring the sample that you want to be tested, we will see a variety of prokaryotic and eukaryotic microorganisms swimming about. Both types are present and it's a matter of identifying one from the other.
Theyre all kinds of different sizes and shapes.
A hay infusion is a culture made from water collected from a pond, lake, stream or puddle.
Infusion
Hay infusion consists of microorganisms like protozoa. Most of the protozoa are motile in nature since they have locomotive structures like flagella, cilia and pseudopods that's why the organisms that can be found in hay infusion generally moves.
Hay infusion is a method of making a suitable home for your microorganisms to grow, this is achieved by boiling hay with water. This results to a broth, rich with carbohydrates and other "food" for your microorganisms extracted from the hay you used. Now, to make your microorganisms grow in numbers in that infusion, you need to get a little amount of the sample that you want to test for the presence of microorganisms. Example, getting a few milliliters of pond water, then pouring it to your hay-infusion. We will leave this for 24-48 hours so that the microorganisms will be able to multiply and grow in your infusion. Now, regarding the question on prokaryotic and eukaryotic cells... These two are actually the types of cells animals may have. Prokaryotic cells are said to be primitive and are commonly seen in bacteria, while eukaryotic cells are more advanced cells seen in higher forms of life like us. However, in the infusion you prepared, after pouring the sample that you want to be tested, we will see a variety of prokaryotic and eukaryotic microorganisms swimming about. Both types are present and it's a matter of identifying one from the other.
Theyre all kinds of different sizes and shapes.
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In a wet mount the microorganisms are hard to see because they blend in with their surroundings or the liquid they are grown in. An example of this would be in a hay broth where the water is cloudy and the microorganisms are transparent. However, if the microorganisms are being viewed as a stained preparation they will stand out and you will be able to see their size and shape much easier.
oxygen breathers
A hay infusion is a culture made from water collected from a pond, lake, stream or puddle.
Infusion
Bacteria grow better in the dark.
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They are present as cysts on the surface of the infusion material. Wetting the cysts liberates the organisms, which grow, divide and re-encyst as a part of their life cycles. Many of them are present in the air we breathe as cysts as well.
They are hard to see because they are not stained so they blend into their surroundings.