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Heat rises. If the neck of your tube is hot, the air around it is rising away from the top of the tube. It is less likely to become contaminated with microbes you aren't interested in. Prokaryotes are ubiquitous. Sterile technique is critical if you want to study them.

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13y ago
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15y ago

To kill any bacteria on the rim of the tube. This would contaminate a culture if a loop is touched to the side of the glass tube near the top.

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12y ago

It directs the air up and away from the tube preventing environmental contamination of the open tube

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13y ago

this process sterilizes the tube

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Q: Why should you flame the necks of your tubes in microbiology?
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