You should first focus the slide on low power before you switch it to high power because it is easier to find the specimen on the slide. When you switch to a higher power, the field of view gets zoomed in.
first, because you don't want to break the slide.
but usually because it's easier to find what you want on the slide and also easier to focus. use the macro/course focus, change to a high power like 40x, and then use the fine focus.
for all the above reasons, this can be critical if you want to see something in a 100x objective with oil immersion.
It is not strictly necessary, but it is a good practice because suddenly switching from low to high power can be disorienting. Going slowly from one magnitude to the next prevents you from getting confused at what you are looking at or having to search and refocus a lot to find what you are looking for.
The specimen should be in clear focus
Because it is too hard to find a specimen on high power. Easier to find them and focus them on low power and then increase the magnification and fine-tune the focus to get a better, closer look.
The coarse adjustment knob should never be used when viewing in high power with a compound microscope. When in high power, use the coarse adjustment (the knob smaller than the coarse adj.) to more accurately focus on the subject.
Because high power takes up a much smaller area, and you may not be able to find the specimen if it's off to one side. C:
You use the low power objective lens first to get your sample centered in the field of view.
Fine focus
WHY IS IT IMPORTANT THAT THE IMAGE IS CENTERED IN THE FIELD OF VIEW BEFORE SWITCHING TO A HIGHER POWER
I don’t know
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The low power objective lens is usually a 10x lens and it is used to orient the specimen and get it focused before switching to a higher power lens.
You can see what you are doing better in low power in high power, so you can center the object first before you switch to high power to make minor adjustments.
It is to open the power-switch at zero current to minimize the looses during switching.
The low power objective lens is usually a 10x lens and it is used to orient the specimen and get it focused before switching to a higher power lens.
lowest power
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A switching power supply adapter from Dee Van Electronics.