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You should first focus the slide on low power before you switch it to high power because it is easier to find the specimen on the slide. When you switch to a higher power, the field of view gets zoomed in.

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9y ago
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13y ago

first, because you don't want to break the slide.

but usually because it's easier to find what you want on the slide and also easier to focus. use the macro/course focus, change to a high power like 40x, and then use the fine focus.

for all the above reasons, this can be critical if you want to see something in a 100x objective with oil immersion.

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11y ago

It is not strictly necessary, but it is a good practice because suddenly switching from low to high power can be disorienting. Going slowly from one magnitude to the next prevents you from getting confused at what you are looking at or having to search and refocus a lot to find what you are looking for.

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Q: Why should you focus on low power before switching to high power?
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What should you expect to change after switching to a higher power?

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