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Why spectrofluorometry potentially more sensitive than spectrophotometry?
Wiki User
∙ 13y ago
Due to luminescence (i.e fluorescing capability of molecule), which is inherently more sensitive then absorption. Imagine dark stadium, 1 flashlight turns on. You can see it. That's luminescence. Now imagine the stadium with 500 flashlights on and 1 gets turned on. You won't really notice, i.e. less sensitive, like absorption.
In luminescence and spectrofluorometry the background is dark with all the other non-fluorescing molecules. The ones the fluoresce are clearly seen and can be detected to individual molecules.
Wiki User
∙ 13y ago
Wiki User
∙ 7y agoFlluorescence spectrophotometry is selective. A fluorescent molecule usually has a unique excitation and emission wavelength. It is commonly used as a detector for HPLC. It is also often used in research for cell-based assays.
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Is the tongue or finger more sensitive?
The finger is more sensitive to touch. The tongue is more sensitive to temperature. Test it out for your self!
Rods are less light sensative and more color sensitive than are cones?
Rods are more light-sensitive and less color-sensitive than are cones.
What is more sensitive sight or hearing?
hearing by far
What area of the eye is most sensitive to light?
The part of your eye that is most sensitive to light is the retina. This is not because the retinal tissue is any more fragile than other tissue in the eye, but because the cornea and lens focus incoming light onto the retina, potentially magnifying it as much as 100,000 times. The result is that bright light that would not damage any other part of the eye can severely damage the retina.
Cells of the conducting system in the heart is more sensitive to which ion?
potassion ion
What is the comparative and superlative of the word sensitive?
Comparative: more sensitive Superlative: most sensitive
Are teenage ears less sensitive then adults?
No. They are more sensitive
What is the comparative and superlative of sensitive?
comparative -- more sensitive superlative -- most sensitive
Why men nipples sensitive?
The mans nipple is a bit sensitive , but the lady will have a far more sensitive nipple.
Are your feet more sensitive then your hands?
False. There are more sensitive touch receptors on the tip of your finger than on your back
Why is your dog more ticklish now?
He/she has probably become more sensitive. This can happen for a number of reasons - for example, a bitch can be more sensitive during her seasons.
Why is your hand more sensitive than your non-dominant hand?
It is more sensitive because your dominant hand is used the most and therefore it is the most sensitive also there are nerve endings in your hand.
Would an obese person be more of less sensitive to temperature change?
In general : less sensitive to cold, but more sensitive to heat. It has to do with the insulating properties of fat, and surface to volume ratios.
How much more sensitive are animals hearing compared to humans?
humans ears are more sensitive because animals have super hearing
Are shetland ponies sensitive.?
it depends on what you are talking about. all horses have feelings so they can get sensitive on what you do or say to them. but their skin can get sensitive. different breeds of horses have more or less sensitive skin.
Are gay men sensitive?
No more (or less) sensitive than members of other minority groups.
Are cells that divide rapidly more sensitive to radiation?
No, they are just as sensitive as any other cell.
