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Why you are using enzyme blank and substrate blank instead of using a single blank to find out the enzyme activity?
I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?
What else can I help you with?
How many substrate molecules does a typical enzyme act on per second?
A single enzyme molecule can act on about 1000 substrate molecules per second.
How many substrates can an enzyme have?
An enzyme can have multiple substrates, as it can bind to more than one substrate molecule at a time. This binding can occur at the active site of the enzyme, where the substrates interact with the enzyme's catalytic residues to facilitate the chemical reaction. The specificity of the enzyme's active site determines which substrates can bind to the enzyme.
How is a specific enzyme able to catalyze a specific reaction in the cytoplasm of a single cell with many different enzymes?
Enzymes have specific active sites that bind to specific substrate molecules, allowing them to catalyze specific reactions. Each enzyme has a unique shape that fits specific substrates like a lock and key. This specificity enables the enzyme to function in the cytoplasm of a cell with many other enzymes.
How enzyme lowering the activation energy?
Enzymes lower activation energy by providing the substrates with an ideal environment for the certain reaction. Often, certain parts of the enzyme chain in the activation site will make the substrate more unstable by binding to it. This instability makes it easier to break down the substrate, or attach another molecule to it.
What are the two types of Feedback Inhibition?
In my understanding there are three types of feedback inhibition:SIMPLE: Enzyme inhibited by single end product.CUMULATIVE: More than one end product inhibits the same enzyme. That means that each product exerts partial inhibition and inhibition is cumulative.CONCERTED: More than one end product must bind the same enzyme simultaneously for any inhibition.I !
How many substrate molecules does a typical enzyme act on per second?
A single enzyme molecule can act on about 1000 substrate molecules per second.
How many substrates can an enzyme have?
An enzyme can have multiple substrates, as it can bind to more than one substrate molecule at a time. This binding can occur at the active site of the enzyme, where the substrates interact with the enzyme's catalytic residues to facilitate the chemical reaction. The specificity of the enzyme's active site determines which substrates can bind to the enzyme.
How fast do enzymes act?
Enzymes reaction cycle is so fast that a single enzyme molecule typically act on about thousand substrate molecules per second.
How is a specific enzyme able to catalyze a specific reaction in the cytoplasm of a single cell with many different enzymes?
Enzymes have specific active sites that bind to specific substrate molecules, allowing them to catalyze specific reactions. Each enzyme has a unique shape that fits specific substrates like a lock and key. This specificity enables the enzyme to function in the cytoplasm of a cell with many other enzymes.
Do enzymes work only on single substrate in most cases?
Yes, almost all work on a single substrate.
How enzyme lowering the activation energy?
Enzymes lower activation energy by providing the substrates with an ideal environment for the certain reaction. Often, certain parts of the enzyme chain in the activation site will make the substrate more unstable by binding to it. This instability makes it easier to break down the substrate, or attach another molecule to it.
Result of mixing both enzymes with there substrates in a single test tube?
Mixing both enzymes with their substrates in a single test tube could result in the enzymes catalyzing their respective reactions simultaneously. This may lead to the production of different products depending on the specificity of each enzyme for its substrate. However, it is important to consider factors such as pH, temperature, and compatibility of the enzymes to ensure proper activity and prevent any interference between the reactions.
What are the two types of Feedback Inhibition?
In my understanding there are three types of feedback inhibition:SIMPLE: Enzyme inhibited by single end product.CUMULATIVE: More than one end product inhibits the same enzyme. That means that each product exerts partial inhibition and inhibition is cumulative.CONCERTED: More than one end product must bind the same enzyme simultaneously for any inhibition.I !
Difference between activator and prosthetic groups?
Prosthetic groups can be as simple as a single metal ion bound into the enzyme's structure, or may be a more complicated organic molecule (which might also contain a metal ion). it is permanently bonded to enzyme. Activator is only metal ion that is detachable. source:chemguide.co.uk Stuffidious.com
What is the scientific name of enzymes?
Enzymes belong to a class of proteins called "biocatalysts" and do not have a single scientific name. They are named based on the substrate they act upon and end with the suffix "-ase." For example, the enzyme that breaks down proteins is called protease.
Do enymes brake down lipids carbohydrates and prptiens?
Yes specific enzymes are breaking down carbohydrates, proteins and lipids to form sugars, amino acids and fatty acids respectively by hydrolysis. All enzymes are specific for a particular substrate. Not a single enzyme can do all these processes!
Does the smooth endoplasmic reticulum have a single or double membrane?
yeh it is a double membrane bound organelle. the inner membrane is heavily folded for a large surface are for enzyme activity. its function is the synthesis, modification and distribution of protiens.
