Advantages and disadvantages of reverse phase liquid chromatography?

Answer 1

One of the advantages of gas chromatography is the ability to identify individual components and the concentrations of each of these components. Another advantage is only a small sample is needed. A disadvantage is that it is limited to volatile samples and it is not suitable for thermally labile samples.

Answer 2

Chromatography is used in chemistry to separate mixtures. In particular, Gas chromatography is used to separate and analyze compounds that don't decompose when vaporized. This allows for separation and analysis of materials that might not normally dissolve in water like normal chromatography uses, but it also requires that the compound not break down when turned into a gas or vapor.

Answer 3

aDVANTAGES

This method can rapidly determine the number of constituents of a mixture sample. Sometimes, paper chromatography even allows to completely identify constituents. Another advantage of this method is that it requires a relatively small sample and is less expensive.

More importantly, paper chromatography doesn't require a specially trained person to perform the method any one can easily carry out the method by following simple instructions and analyse the outcome from the paper chromatogram.

When it comes to separating amino acids proteins, lipids and carbohydrates paper chromatography is quite effective by the fact that:

*It can rapidly determine the number of constituents of a mixture sample. Sometimes, paper chromatography even allows to completely identify constituents. Another advantage of this method is that it requires a relatively small sample and is less expensive.

*Sample preparation is simplified because plates are disposable

Answer 4

Advantages of reverse phase liquid chromatography include high resolution, wide applicability to various compounds, and good sensitivity. However, it can be time-consuming due to long run times, and non-polar compounds may not separate well.

Answer 5

its used to separate low molecular weight molecules and polar compounds

Answer 6

Gas chromatography is accurate for quantification, has a high separating power, is easily automated, and its mobile phase is non-toxic and inexpensive.

Answer 7

It can be