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What is the nam of petri dish gel?
The substance used in petri dishes is called agar agar. It is a gelatinous substance derived from seaweed and is used as a medium for growing microorganisms in laboratory settings.
What is the principle of agar diffusion method?
The principle of agar diffusion method is based on the diffusion of an antimicrobial agent from a sample into an agar medium containing a test organism. The size of the zone of inhibition that forms around the sample is used to determine the effectiveness of the antimicrobial agent against the test organism.
What is the definition of semi-solid agar medium?
Semi-solid agar media have been successfully used for primary culture and drug sensitivity tests of tubercle bacilli directly from sputum homogenates. I really wanna have sex with my friend but i don't know how to start!
What is the chemical composition of malt extract agar?
Malt extract agar typically consists of malt extract, agar as a solidifying agent, and may also contain additional nutrients like peptone or yeast extract. The exact composition can vary depending on the manufacturer or specific recipe used.
Why was agar cooled below 50 degrees celsius?
Agar is cooled below 50 degrees Celsius to prevent it from solidifying too quickly. This allows time for the agar to be poured into Petri dishes and to evenly distribute any added nutrients or samples before it solidifies. Cooling it slowly also helps to avoid the formation of air bubbles in the agar.
What is the colour of klebsiella on salmonella-shigella agar?
Its color is red.
Where can you find a picture of salmonella on nutrient agar?
Search "salmonella culture" then select Images.
How does salmonella appear in agar?
in nutrient agar:- 2-3mm, grey colonies,moist circular,convex colonies are seen.in blood:-grey colonies.in macConkey agar:-pale /yellowcolonies are seen.in deoxycholatecitrate agar:-colourless,smooth,shiny colonies salmonella are seen.
Can salmonella typhimurium grow on mannitol salt agar?
No, Salmonella Typhimurium cannot grow on Mannitol Salt Agar because it is a selective and differential media primarily used for isolating and differentiating Staphylococcus species based on their ability to ferment mannitol. Salmonella Typhimurium is not a mannitol fermenter, so it will not grow on this agar.
What is the color of nutrient agar?
Nutrient agar is a clear pale buff colour.
List selective media for stool culture?
MacConkey agar: Selective for Gram-negative bacteria, inhibits the growth of Gram-positive bacteria. Hektoen enteric agar: Selective for Gram-negative enteric pathogens, such as Salmonella and Shigella species. Thiosulfate-citrate-bile salts-sucrose (TCBS) agar: Selective for Vibrio species, especially Vibrio cholerae. Cefsulodin-irgasan-novobiocin (CIN) agar: Selective for Yersinia species.
Why is salmonella shigella agar not autoclaved?
Salmonella Shigella (SS) agar is not autoclaved because it is a selective culture medium designed to isolate and differentiate Salmonella and Shigella species from other bacteria in clinical and environmental samples. Autoclaving could degrade key components of the medium, such as bile salts and certain nutrients, which are crucial for the selective growth of the target organisms. Instead, SS agar is typically sterilized using filter sterilization to maintain its effectiveness while preventing contamination.
What does SMID2 stand for in microbiology?
SMID means "Salmonella Identification." It is an agar made by bioMerieux that contains chemicals which change color when salmonella is grown on the plate. SMID2 is the new-and-improved version.
How do you differentiate biochemical results between e coli and salmonella?
To differentiate between E. coli and Salmonella, key biochemical tests can be utilized. E. coli typically ferments lactose, producing acid and gas in tests like lactose broth and MacConkey agar, while Salmonella does not ferment lactose and often appears colorless on MacConkey agar. Additionally, Salmonella produces hydrogen sulfide (H2S) on triple sugar iron (TSI) agar, resulting in a black precipitate, whereas E. coli does not. Further biochemical tests, such as urease and indole tests, can also help in distinguishing the two.
How does samonella and shigella appear on S.S agar?
Shigella and the majority of salmonellae Clear, colorless, transparent Salmonella enteriditis ATCC 13076 Colorless with black center Salmonella typhi ATCC 6539 Colorless with black center Salmonella typhimurium ATCC 14028 Colorless with black center
Can serratia marcescens grow in mannitol salt agar?
No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.
Which bacteria gives dark puple colour on EMB agar medium?
Escherichia coli is a bacterium that can produce a dark purple color on Eosin Methylene Blue (EMB) agar medium due to its ability to ferment lactose and produce acidic end products. This characteristic color change helps in the differentiation and identification of E. coli from other bacteria.
