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Absorption method measures the amount of light absorbed by a sample at a specific wavelength, and is used to quantify the concentration of a substance. Colorimetric method utilizes color changes to detect the presence or concentration of a substance, often involving a chemical reaction that produces a color change.
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Why protein estimation is done at 660 nanometer in folin lowry method?
Protein estimation in the Folin-Lowry method is done at 660 nanometers because this wavelength corresponds to the highest absorption peak of the complex formed between proteins and the Folin-Lowry reagent. This wavelength is ideal for accurately measuring the concentration of proteins present in a sample based on the colorimetric reaction that occurs.
Why is electrometric method of pH determination more accurate than colorometric method?
The electrometric method of pH determination provides a single value dependent on the concentration of hydrogen ions in the aqueous solution being tested. The colorimetric method of pH determination uses acid-base indicators to provide a range of values for a solution's pH, and is therefore less accurate than the electrometric method.
How do you determine the colorimetric estimation of unknown copper sulphate concentration in solution?
To determine the colorimetric estimation of unknown copper sulfate concentration in solution, you can use a colorimetric method such as a complexation reaction with a specific reagent that forms a colored complex with copper ions. By measuring the absorbance of this colored complex at a specific wavelength using a colorimeter, you can correlate the absorbance values to known copper sulfate concentrations to determine the concentration of the unknown solution. Calibration curve plotting absorbance against known concentrations is commonly used in colorimetric estimations.
What is colorimetric titration?
The term is sometimes applied titrations where the end point is determined by a color change- e.g. using ssy methyl orange. Sometimes the term is used when a colorimeter is used- the absorption at a particular frequency of light is used to determine the concentration- (Beers Law)
How can one accurately measure CO2 levels in water?
One can accurately measure CO2 levels in water using a pH meter or a colorimetric method. pH meters measure the acidity of the water, which can indicate the presence of CO2. Colorimetric methods involve adding a reagent that changes color in the presence of CO2, allowing for visual measurement of the levels.
What is a blanket overhead absorption rate How will you differentiate between blanket overhead rate and other overhead absorption rate?
In Blanket Overhead Absorption Rate applied is the same however it may differ if a company follow Departmental method Or frther break - up method
Why we need colorimetric method in thermodynamics?
a proper question would be: Why do we need a calorimetric method in thermodynamics ?Then the answer would be: to measure the heat-capacity.
Why protein estimation is done at 660 nanometer in folin lowry method?
Protein estimation in the Folin-Lowry method is done at 660 nanometers because this wavelength corresponds to the highest absorption peak of the complex formed between proteins and the Folin-Lowry reagent. This wavelength is ideal for accurately measuring the concentration of proteins present in a sample based on the colorimetric reaction that occurs.
What is overhead absorption rate. What are its purpose?
method of overhead absorption
Why is electrometric method of pH determination more accurate than colorometric method?
The electrometric method of pH determination provides a single value dependent on the concentration of hydrogen ions in the aqueous solution being tested. The colorimetric method of pH determination uses acid-base indicators to provide a range of values for a solution's pH, and is therefore less accurate than the electrometric method.
What is fiske subbaRow procedure?
It's method of determining the inorganic phosphate content in urine or blood, etc. It is a colorimetric assay.
Differentiate analytic method from synthetic method in maths?
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What is absorption method of marginal costing?
Absorption method is that in which predetermined overhead rate is use to allocate all overheads to departments or activities.
How can an icteric hemolyzed or lipemic sample be corrected for total protein assay?
An icteric sample can be corrected by using a method that does not rely on colorimetric detection, such as turbidimetric or refractometric methods. Hemolysis can be corrected by using a spectrophotometric method that measures protein concentration at a wavelength where hemoglobin absorption is minimal. Lipemia can be corrected by using ultracentrifugation or filtration to remove the lipids before analyzing the sample for total protein.
Which are the Methods for hemoglobin estimation?
Hemoglobin estimation is the measuring of hemoglobin levels that are broken into three categories. Visual methods are Sahil, Dares, Hadens, Wintrobes, Haldanes, Tallquists method. Second to consider are the gasometric method, single to the Van Slyke method. Lastly the spectrophotometric method oxyhemoglo, and the cyanmethemoblobin method.
How do you determine the colorimetric estimation of unknown copper sulphate concentration in solution?
To determine the colorimetric estimation of unknown copper sulfate concentration in solution, you can use a colorimetric method such as a complexation reaction with a specific reagent that forms a colored complex with copper ions. By measuring the absorbance of this colored complex at a specific wavelength using a colorimeter, you can correlate the absorbance values to known copper sulfate concentrations to determine the concentration of the unknown solution. Calibration curve plotting absorbance against known concentrations is commonly used in colorimetric estimations.
What is colorimetric titration?
The term is sometimes applied titrations where the end point is determined by a color change- e.g. using ssy methyl orange. Sometimes the term is used when a colorimeter is used- the absorption at a particular frequency of light is used to determine the concentration- (Beers Law)
