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How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
How to read an HPLC chromatogram effectively?
To read an HPLC chromatogram effectively, start by identifying the peaks representing different compounds. Analyze the peak shapes, heights, and retention times to determine the compounds present. Compare the chromatogram to a standard or reference for accurate identification. Pay attention to any abnormalities or unexpected peaks that may indicate issues with the analysis.
What is the application of histamine chamber?
A histamine chamber is used to test for histamine sensitivity or allergies by exposing the individual to controlled levels of histamine and observing their reaction. This type of test can help diagnose conditions like histamine intolerance or allergies.
What are the key differences between normal phase HPLC and reverse phase HPLC in terms of their separation mechanisms and applications?
Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.
What substance produced by the body is counteracted by anthistamine drugs?
Histamine is produced by the body in response to allergens, and antihistamine drugs work by blocking the action of histamine in the body.
What can one read in the HPLC Column?
In an HPLC column one can see very small molecules such as ATP, histidine, glucose, uracil, and pyridine. It is a form high quality of liquid Chromatography.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What do basophils release?
Histamine and heparin
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
Do neutrophils produce histamine?
no it is the basophils that produce histamine
How to read an HPLC chromatogram effectively?
To read an HPLC chromatogram effectively, start by identifying the peaks representing different compounds. Analyze the peak shapes, heights, and retention times to determine the compounds present. Compare the chromatogram to a standard or reference for accurate identification. Pay attention to any abnormalities or unexpected peaks that may indicate issues with the analysis.
What is the application of histamine chamber?
A histamine chamber is used to test for histamine sensitivity or allergies by exposing the individual to controlled levels of histamine and observing their reaction. This type of test can help diagnose conditions like histamine intolerance or allergies.
During an allergic reaction what chemical is released?
histamine
Which granulocyte produces histamine?
Basophils are the granulocytes that produce and release histamine. Histamine is involved in the inflammatory response and allergic reactions.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
What is histamine chamber?
Histamine Chamber is a scientific instrument use to evaluate antiasthmatic activity of certain drugs/chemicals in lab. animals. The Lab. animals are exposed to histamine aerosol in the Histamine Chamber.
