Function of GTE buffer solution

Answer 1

GTE stands for Glucose-Tris-EDTA.Glucose is used to maintain osmolarity: 50mM (millimolar) glucose prevents premature cell lysis, which can cause lower DNA yields to due to aggregation and degradation. The other components of the buffer also contribute to the osmolarity of the solution, but glucose, being a non-electrolyte, is a good choice because it does not interfere with the solution's buffer properties.

The Tris is used to buffer whatever you're adding this to at pH 7.9. EDTA binds divalent cations like Ca2+ and Mg2+, thereby weakening the cell envelope of cells in the mixture. This is typically used for miniprep/DNA purification, when you have to lyse the cell and get internal cell components out into the solution.

Answer 2

A GTE buffer solution is used to stabilize the pH level during nucleic acid extraction procedures, such as RNA isolation. It helps to maintain the integrity of the nucleic acids by providing a suitable environment for the enzymes involved in the extraction process. Additionally, the GTE buffer solution helps to prevent degradation of nucleic acids by RNases (ribonucleases).