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The spectrophotometer is an instrument that measures the amount of light of a specified wavelength which passes through a medium. This instrument operates by passing a beam of light through a sample and measuring the intensity of light reaching a detector.
Wiki User
∙ 9y ago
Wiki User
∙ 15y agoA spectrophotometer (or spec as it is generally called), measures the absorbance of a sample at a particular wavelength set by the user. The user has to choose the wavelength maxima of the sample and input this value before taking any readings.
A blank solution has to be prepared - one that contains all the sample components except the analyte. The instrument measures the absorbance of this blank and assigns it a value of zero. Sample reading are taken with reference to this zero setting.
The absorbance values that are obtained from samples are also referred to as the optical density
Wiki User
∙ 14y agospectrophotometer is a machine which tells about the growth of microorganisms in culture by examining the colour change.
Wiki User
∙ 12y agoto plot the calibration curve and to get the slope and the molar absorptivity coefficient that can be use to determine the concentration of the solute.
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What are the function of NaOH in the experiment of determination of solute concentration in solution using spectrophotometer?
NaOH is added to p-nitrophenol to change it into p-nitropenyl, which can give the maximum Absorption.
How is spectrophotometer important in the clinics?
Spectrophotometer is portable clinic infra-red machine for medical analysis. It can be used for pathway analysis or for comparison with genetic data.
Blood component that can be measured by spectrophotometer?
Iron, from hemoglobin
Who invented the spectrophotometer?
Robert fruen
Calibrated the spectrophotometer with?
Spectrophotometric standards are used - crystals or solutions.
What is the function of the spectrophotometer?
spectrophotometer is used to determine the concentration of solution by means of reflectance or transmittance by the solution
How do you work spectrophotometer?
. A spectrophotometer is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color, or more specifically, the wavelength of light
What is the function of detector in UV spectrophotometer?
Detector converts optical signal to electric signal
What are the two main functions of a spectrophotometer?
A spectrophotometer is an instrument to measure how much light of a specified wavelength passes through a medium. It quantitatively measures the reflection or transmission properties of a material as a function of wavelength. The main uses of spectrophotometer are in the forensic sciences (including chemistry, biology, biochemistry, physics, etc.) as well as the medical field with the purpose being research.
What is the function of a spectrophotometer in a microbiology lab?
To magnify the specific microbes that are being viewed. Most microbes you cannot see with the naked eye.
What wavelength measures.?
spectrophotometer
What instrument is used to measure the amount of chemical of a solution based on its color?
spectrophotometer
What are the function of NaOH in the experiment of determination of solute concentration in solution using spectrophotometer?
NaOH is added to p-nitrophenol to change it into p-nitropenyl, which can give the maximum Absorption.
Why you are using kcl in the calibration of uv visible spectrophotometer?
how do you determine degree of deacetylation of chitosan using UV -vis spectrophotometer
Where can I get a Spectrophotometer fixed in Southwest Florida?
Currently, there are no places that are in FL that can fix them, however, you can call the number on the back of the Spectrophotometer & they can assist you.
Can a spectrophotometer be used to diagnose diabetes?
no
How would a spectrophotometer be used in treating diabetes?
A spectrophotometer is not and could not be used to treat diabetes. This is a device that measures light in a specific wavelength.
