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A. Ziehl-Neelsen method for acid-fast staining (6, 7)
Carbolfuchsin stain:
Basic fuchsin, 0.3 g
Ethanol, 95% (vol/vol), 10 ml
Phenol, heat-melted crystals, 5 ml
Distilled water, 95 ml
Dissolve the basic fuchsin in the ethanol; then add the phenol dissolved in the water.
Mix and let stand for several days. Filter before use.
Decolorizing solvent:
Ethanol, 95% (vol/vol), 97 ml
Hydrochloric acid (concentrated), 3 ml
Counterstain:
Methylene blue chloride, 0.3 g
Distilled water, 100 ml
B. Kinyoun method for acid-fast staining (15)
Kinyoun carbolfuchsin solution:
Solution A. Dissolve 4 g of basic fuchsin in 20 ml of ethyl alcohol.
Solution B. Dissolve 8 g of phenol (melted) in 100 ml of distilled water.
Mix solutions A and B together and allow to stand for a few days.
Acid-alcohol decolorizing agent:
Ethanol, 95% (vol/vol), 97 ml
Hydrochloric acid (concentrated), 3 ml
Methylene blue counterstain:
Methylene blue chloride, 0.3 g
Distilled water, 100 ml
Dissolve by shaking.
C. Truant method for acid-fast staining (6, 7)
Fluorescent staining reagent:
Auramine O, CI 41000, 1.50 g
Rhodamine B, CI 749, 0.75 g
Glycerol, 75 ml
Phenol (heat melted crystals), 10 ml
Distilled water, 50 ml
Mix the two dyes well with 25 ml of the water and the phenol. Add the remaining water and glycerol and mix again.
Filter the resulting staining fluorescent reagent through glass wool and store at 4oC or room temperature.
Decolorizing solvent:
Ethanol, 70% (vol/vol), 99.5 ml
Hydrochloric acid (concentrated), 0.5 ml
Counterstain:
Potassium permanganate, 0.5 g
Distilled water, 99.5 g
What else can I help you with?
How can I add patina to copper?
To add patina to copper, you can use a combination of household ingredients like vinegar, salt, and ammonia to create a chemical reaction that will oxidize the copper and give it a greenish-blue patina. Simply mix these ingredients together, apply the solution to the copper surface, and let it sit for a few hours or overnight to develop the desired patina.
How do you prepare 0.5 N sulphuric acid?
To prepare 0.5 N sulfuric acid, you can mix 49 g of sulfuric acid (H2SO4) into enough water to make 1 liter of solution. This will give you a solution that has a concentration of 0.5 N, meaning it contains 0.5 moles of H2SO4 per liter of solution. Remember to always add acid to water slowly while stirring to avoid splashing and to ensure a safe preparation process.
What is the percent acidity of the solution?
To find the percent acidity of a solution, divide the amount of acid by the total solution volume and multiply by 100. This will give you the percentage of acidity in the solution.
Preparation of 0.1 normal solution of sodium carbonate in 1 liter?
To prepare a 0.1 N solution of sodium carbonate in 1 liter, dissolve 5.3 grams of anhydrous sodium carbonate (Na2CO3) in distilled water and make up the volume to 1 liter with water. Stir well until the sodium carbonate is completely dissolved. This will give you a 0.1 N solution of sodium carbonate in 1 liter.
What word describes the solution formed?
The word that describes the solution formed would be 'solute.'
What cells give a false impression ABBR?
The cells that give a false impression are often referred to as "artifact cells." These can appear during slide preparation or staining processes, leading to misleading interpretations under a microscope. Common examples include staining artifacts, debris, or improperly prepared cells that do not accurately represent the true cellular composition of the sample. It's essential for pathologists and researchers to recognize these artifacts to avoid misdiagnosis or incorrect conclusions.
How do you change prepare to pronoun?
Prepare is a verb. The noun form of prepare is preparation; the pronoun for preparation is it. Example use:I have a preparation to sooth sunburn; I will give it to you.
What can you do for the dark places under a Maltese eyes caused by her tears staining her white fur?
I think either you give them botteled water or tap, I don't know, but it improved my maltese's staining!
How do you prepare wood for staining?
I sand it with a final sanding of 400 grit paper, then scrub it with 0000 steel wool, wipe it of carefully then give it first staining with a rag dipped in the stain.
Give the professional preparation of a professional nurse?
what preparation is necessary for the test including course work to be a registered nurse
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Can you give me a solution for this?
for what?
What are the characteristec of a solution?
give me one characteristic of a solution in science
What preparation does a lawyer give you for other jobs?
However much you pay them...
What is the role of calcium chloride in the preparation of media?
they are teir to give blowjobs
Preparation of 0.1n iodine solution?
According to Kodak: For a 0.1 Normal (0.1N) solution of iodine (I2), in 1 L volume flask, dissolve 40 g potassium iodide in 25 mL water. Next, add 12.7 g iodine (I2) and dilute to 1 L. Note that a 0.1N solution is not the same as a 0.1M solution. For iodine 0.1N is 0.05M.
