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Give the ingredients and preparation of the acid fast staining solution?
Wiki User
∙ 10y ago
A. Ziehl-Neelsen method for acid-fast staining (6, 7)
Carbolfuchsin stain:
Basic fuchsin, 0.3 g
Ethanol, 95% (vol/vol), 10 ml
Phenol, heat-melted crystals, 5 ml
Distilled water, 95 ml
Dissolve the basic fuchsin in the ethanol; then add the phenol dissolved in the water.
Mix and let stand for several days. Filter before use.
Decolorizing solvent:
Ethanol, 95% (vol/vol), 97 ml
Hydrochloric acid (concentrated), 3 ml
Counterstain:
Methylene blue chloride, 0.3 g
Distilled water, 100 ml
B. Kinyoun method for acid-fast staining (15)
Kinyoun carbolfuchsin solution:
Solution A. Dissolve 4 g of basic fuchsin in 20 ml of ethyl alcohol.
Solution B. Dissolve 8 g of phenol (melted) in 100 ml of distilled water.
Mix solutions A and B together and allow to stand for a few days.
Acid-alcohol decolorizing agent:
Ethanol, 95% (vol/vol), 97 ml
Hydrochloric acid (concentrated), 3 ml
Methylene blue counterstain:
Methylene blue chloride, 0.3 g
Distilled water, 100 ml
Dissolve by shaking.
C. Truant method for acid-fast staining (6, 7)
Fluorescent staining reagent:
Auramine O, CI 41000, 1.50 g
Rhodamine B, CI 749, 0.75 g
Glycerol, 75 ml
Phenol (heat melted crystals), 10 ml
Distilled water, 50 ml
Mix the two dyes well with 25 ml of the water and the phenol. Add the remaining water and glycerol and mix again.
Filter the resulting staining fluorescent reagent through glass wool and store at 4oC or room temperature.
Decolorizing solvent:
Ethanol, 70% (vol/vol), 99.5 ml
Hydrochloric acid (concentrated), 0.5 ml
Counterstain:
Potassium permanganate, 0.5 g
Distilled water, 99.5 g
Wiki User
∙ 10y ago
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