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To calculate the isoelectric point using 3 pKa values, find the average of the two pKa values closest to the pH at which the molecule carries no net charge.

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How to calculate the isoelectric point using three pKa values?

To calculate the isoelectric point using three pKa values, find the average of the two pKa values closest to the pH at which the molecule carries no net charge.


What is the method used to calculate the isoelectric point of a molecule?

The isoelectric point of a molecule is calculated using the average of the pKa values of its ionizable groups. This point represents the pH at which the molecule carries no net charge.


How can one calculate pi in biochemistry?

Calculating pi in biochemistry involves determining the isoelectric point of a molecule, which is the pH at which the molecule carries no net electrical charge. This can be done by considering the pKa values of the molecule's ionizable groups and using a mathematical formula to calculate the isoelectric point.


How to calculate the pI of a peptide?

To calculate the isoelectric point (pI) of a peptide, you need to determine the average of the pKa values of its ionizable groups. This can be done using online tools or by manually calculating the pI based on the amino acid sequence and their respective pKa values.


How can one determine the isoelectric point (pI) of an amino acid?

The isoelectric point (pI) of an amino acid can be determined by finding the pH at which the amino acid has no net charge. This can be done by calculating the average of the pKa values of the amino acid's ionizable groups, or by using a graph to find the pH at which the amino acid is neutral.


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