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The concentration of ascorbic acid in an unknown sample can be obtain by conducting a few steps by using the DCPIP test. Firstly, we must obtain the volume (dm ) of the sample solution which are required to turn the DCPIP solution (blue) colour into colourless. After obtaining the volume we use an electronic balance to obtain the mass of the sample solution in gram (g). Then we use the concentration formula of [mass (g) of solute per volume (dm ) of solution] to find out the concentration. Hence. The concentration obtained was the concentration of ascorbic acid.
What else can I help you with?
How can one calculate the unknown concentration from a standard curve?
To calculate the unknown concentration from a standard curve, you first need to plot a graph with known concentrations and corresponding measurements. Then, you can use the equation of the line to determine the concentration of the unknown sample based on its measurement.
How can one determine the concentration of a sample using a calibration curve?
To determine the concentration of a sample using a calibration curve, you first need to measure the response of known standard samples with known concentrations. Then, plot a calibration curve by graphing the response against the concentration. Finally, measure the response of the unknown sample and use the calibration curve to determine its concentration by finding where its response falls on the curve.
Why is a titration performed?
Titration is performed to determine the concentration of a substance in a solution. It involves reacting two solutions - one with a known concentration and the other with an unknown concentration - until they reach an equivalence point, allowing for the calculation of the unknown concentration.
What is iodimetry?
Iodimetry is a type of volumetric analysis that involves the use of iodine as a titrant to determine the concentration of an analyte (substance being analyzed) in a solution. It is commonly used in chemistry to determine the amount of substances such as ascorbic acid, thiosulfate, or hydrogen peroxide in a sample.
How can the calibration curve of absorbance versus concentration be used to determine the concentration of a substance in a sample?
The calibration curve of absorbance versus concentration can be used to determine the concentration of a substance in a sample by measuring the absorbance of the sample and comparing it to the absorbance values on the calibration curve. By finding the corresponding concentration value on the curve, the concentration of the substance in the sample can be determined accurately.
How do you determine the concentration of ascorbic acid in unknown sample?
first measure the volume of the sample solution needed to change the blue color of the DCPIP solution into colourless. then, weigh the mass of the sample solution. finally calculate the concentration by using the formula: volume required t change the color of DCPIP solution (dm) per mass of the sample solution (g)
How can one calculate the unknown concentration from a standard curve?
To calculate the unknown concentration from a standard curve, you first need to plot a graph with known concentrations and corresponding measurements. Then, you can use the equation of the line to determine the concentration of the unknown sample based on its measurement.
How can one determine the concentration of a sample using a calibration curve?
To determine the concentration of a sample using a calibration curve, you first need to measure the response of known standard samples with known concentrations. Then, plot a calibration curve by graphing the response against the concentration. Finally, measure the response of the unknown sample and use the calibration curve to determine its concentration by finding where its response falls on the curve.
Why is a titration performed?
Titration is performed to determine the concentration of a substance in a solution. It involves reacting two solutions - one with a known concentration and the other with an unknown concentration - until they reach an equivalence point, allowing for the calculation of the unknown concentration.
What is iodimetry?
Iodimetry is a type of volumetric analysis that involves the use of iodine as a titrant to determine the concentration of an analyte (substance being analyzed) in a solution. It is commonly used in chemistry to determine the amount of substances such as ascorbic acid, thiosulfate, or hydrogen peroxide in a sample.
How can the calibration curve of absorbance versus concentration be used to determine the concentration of a substance in a sample?
The calibration curve of absorbance versus concentration can be used to determine the concentration of a substance in a sample by measuring the absorbance of the sample and comparing it to the absorbance values on the calibration curve. By finding the corresponding concentration value on the curve, the concentration of the substance in the sample can be determined accurately.
How can you calculate absorbance of unknown from linear equation?
To calculate the absorbance of an unknown sample using a linear equation, you first need to establish a calibration curve by plotting the absorbance values of known standards against their concentrations. The resulting linear equation, typically in the form (y = mx + b), relates absorbance (y) to concentration (x), where (m) is the slope and (b) is the y-intercept. By measuring the absorbance of the unknown sample and substituting this value into the linear equation, you can solve for the concentration of the unknown sample. This allows you to determine the absorbance based on its concentration derived from the calibration curve.
How can one utilize a calibration curve to determine the concentration of a substance?
To determine the concentration of a substance using a calibration curve, one must first create the curve by measuring known concentrations of the substance and their corresponding signals. Then, by measuring the signal of an unknown sample and comparing it to the curve, the concentration of the substance can be determined.
How can we accurately determine the protein concentration in a sample using measuring protein concentration techniques?
To accurately determine protein concentration in a sample, techniques such as spectrophotometry, Bradford assay, and BCA assay can be used. These methods involve measuring the absorbance of light by the sample and comparing it to a standard curve to calculate the protein concentration.
Is titration a qualitative or quantitative method?
Titration is a quantitative method used to determine the concentration of a substance in a sample. It involves slowly adding a reagent of known concentration to the solution being analyzed until a specific endpoint is reached, allowing for the calculation of the unknown concentration.
What is hypothesis of a acid base titration?
The hypothesis of an acid-base titration is that the volume of the acid solution needed to neutralize a base solution is stoichiometrically equivalent to the volume of the base solution required to neutralize the acid. This forms the basis for determining the unknown concentration of an acid or base by titration.
What are the application of radial immunodiffusion?
1.used for determining the antigen concentration of unknown sample. 2.to identify the specific viral antigen from the mixed viral sample.
