To prepare methylene blue indicator mix together some methylene blue with a naproxen solution. Then dilute with distilled water. If you would like your mixture to have a deeper blue color then add some juice from boiled cabbage.
Prepare the methyl red indicator by dissolving 0.1 g of methyl
red in 300 mL of 95% ethyl alcohol. Add sufficient purified
water to make 500 mL.
I found this in the Difco Manual
-Richard
chioler@crc.losrios.edu
0,1g of methylene blue + 18,70ml NaOH solution (0,02M) are mixed then the mixture is diluted to 250ml with distilled water.
Methyl orange is prepared from sulfanilic acid and N, N-dimethylaniline. It uses a diazomium coupling reaction to form aromatic diazomium salts.
According to the USP, 100mg of Methyl Red solid into 100mL of Alcohol.
Dissolve 20mg methyl red and 100mg bromocresol green in 100ml 95% ethyl alcohol.
You use methyl red as a ph indicator for for the MRVP test.
5g methyl orange dry dissolved with 100ml distilled water.
Methyl Red Indicator
Methyl Orange indicator is made by dissolving .01 g of the powder into 100 ml of DI water. This gives and indicator for a pH range of 3.2-4.4 and goes from Red to Yellow.
Methyl red is used as a pH indicator or for the identification of some bacteria.
Dissolve 20mg methyl red and 100mg bromocresol green in 100ml 95% ethyl alcohol.
You use methyl red as a ph indicator for for the MRVP test.
5g methyl orange dry dissolved with 100ml distilled water.
Methyl Red Indicator
Methyl red is an adequate indicator.
Methyl Orange is red in an acidic solution.
Methyl is an indicator, when HCl, which is an acid, is added, the solution will turn red.
Phenolphthalein Litmus Red Litmus Blue Universal Indicator -> Most Common Methyl Orange :)
Methyl Orange indicator is made by dissolving .01 g of the powder into 100 ml of DI water. This gives and indicator for a pH range of 3.2-4.4 and goes from Red to Yellow.
By titration with alkaline on methyl-red indicator (pH<6)
It turns the alkali into a red/yellow colour