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You can determine the End Point of a Fermentation in many different ways.
Often the limiting nutrient stops any further growth. Monitoring the nutrients via offline sampling can be done, but is time consuming and with delay.
Most efficient way is to look at the offgas of the fermentation with a process Mass Spectrometer i.e. Prima dB from Thermo Scientific, where the End Point will be clearly indicated. The parameters OUR (Oxygen Uptake Rate) and CER (Carbondioxide Evolution Rate) will show a good trend, and indicate when to transfer to next scaleUp or stop.
The ratio of OUR and CER gives you RQ, the respiratory Quotient, which tells you which Carbon-source the cells are using as feed. At i.e. glucose RQ = 1, and when feeding on stearic acid RQ goes towards 0,7 - indicating that it is time to add more glucose.
As extra benefits, the Prima dB will measure all other components in the offgas, like MeOH, EtOH, CH4, H2S, other secondary metabolites and many more.
What else can I help you with?
How can one determine the reaction quotient in a chemical reaction?
To determine the reaction quotient in a chemical reaction, you need to calculate the concentrations of the reactants and products at a specific point in time. The reaction quotient is calculated using the same formula as the equilibrium constant, but with the concentrations of the reactants and products at that specific point in time. This helps determine whether the reaction is at equilibrium or not.
Why indicator is being used in titration?
An indicator is so you can see when the end point of the reaction occurs, or when the reaction is complete. This can be an acid-base indicator such as methyl orange which determines the end point with a colour change.
How can you determine the maximum velocity of a reaction by calculating Vmax?
To determine the maximum velocity of a reaction, you can calculate Vmax by plotting a graph of reaction rate against substrate concentration and finding the point where the reaction rate levels off. This point represents the maximum velocity that the reaction can achieve under the given conditions.
What is the difference between end point and equilibrium point in chemistry?
In chemistry, an endpoint is the point in a titration where the indicator changes color, signaling that the reaction is complete. On the other hand, an equilibrium point is the point in a reaction where the concentrations of reactants and products remain constant over time, indicating that the forward and reverse reactions are occurring at the same rate.
The end point of a titration indicates the?
The end point of a titration indicates the point at which the reaction has reached stoichiometric equivalence between the titrant and analyte. This is typically signaled by a noticeable change in a physical property, such as a color change in an indicator or a change in pH.
How you can determine the end-point of the permanganometry?
We can determine the end point of permanganometry by using titration.
How can one determine the reaction quotient in a chemical reaction?
To determine the reaction quotient in a chemical reaction, you need to calculate the concentrations of the reactants and products at a specific point in time. The reaction quotient is calculated using the same formula as the equilibrium constant, but with the concentrations of the reactants and products at that specific point in time. This helps determine whether the reaction is at equilibrium or not.
Why indicator is being used in titration?
An indicator is so you can see when the end point of the reaction occurs, or when the reaction is complete. This can be an acid-base indicator such as methyl orange which determines the end point with a colour change.
How can you determine the maximum velocity of a reaction by calculating Vmax?
To determine the maximum velocity of a reaction, you can calculate Vmax by plotting a graph of reaction rate against substrate concentration and finding the point where the reaction rate levels off. This point represents the maximum velocity that the reaction can achieve under the given conditions.
Why titration of weak acids and weak bases are not done?
It is difficult to determine the end point of such a titration, because the titration produces a buffer solution that changes its pH very slowly at the end point, in contrast to reaction between a strong acid and strong base.
What is the difference between end point and equilibrium point in chemistry?
In chemistry, an endpoint is the point in a titration where the indicator changes color, signaling that the reaction is complete. On the other hand, an equilibrium point is the point in a reaction where the concentrations of reactants and products remain constant over time, indicating that the forward and reverse reactions are occurring at the same rate.
What can determine the midpoint?
The midpoint is the point between the beginning and the end, in distance or in time.
The end point of a titration indicates the?
The end point of a titration indicates the point at which the reaction has reached stoichiometric equivalence between the titrant and analyte. This is typically signaled by a noticeable change in a physical property, such as a color change in an indicator or a change in pH.
What is the difference between the end point in a titration and the equivalence point?
Oh, dude, the end point in a titration is when the indicator changes color, indicating the reaction has almost reached completion. The equivalence point, on the other hand, is when the moles of acid equal the moles of base in a reaction. It's like the end point is the flashy showstopper, and the equivalence point is the behind-the-scenes workhorse.
How many hour drive from los angeles?
In order to determine the amount of hours in a trip, there must be a starting point and end point.
Why must an indicator be used in a titrations?
An indicator is used in titrations to show the endpoint of the reaction when the titrant has completely reacted with the analyte. It helps to visually determine when the reaction has reached the equivalence point.
Why we use indicator in the volumetric analysis?
Indicators are used in volumetric analysis to signal the end point of a reaction. They change color at a specific pH or stoichiometric point, indicating when the reaction is complete. This helps in determining the volume of titrant needed for complete reaction with the analyte.
