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To 2 mL of serum, add 100 mcl concentrated ammonium hydroxide and 5 ml of hexane/isoamyl alcohol (98%/2% v/v). Extract and centrifuge for 5 minutes.

Dry under nitrogen stream at 55 degrees celsius.

wash down the sides of the vial with 200 mcl of methanol and dry to completion.

Use the sim mode on gcms and monitor ions: 82,182,303.

Use 303 to calculate.

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