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How do you convert 20mM tris-Hcl to gm?
let we have to calculate wait of 20mM tris-HCL for a solution of 1liter,then formula for molarity is Molarity= weight (in grams)/molecular weight X volume in liter hence 20/1000=wait/121.14X1 wait = 20 X 121.14/1000 (cross multiplication) wait = 2.42gm
What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?
1x PBS buffer typically has a molarity of around 0.01 M. To prepare a 20 mM PBS buffer, you would need to dilute the 1x PBS stock solution with water. For example, to make 1 liter of 20 mM PBS buffer, you would need to mix 2 ml of 1 M PBS stock solution with 98 ml of water.
How do you prepare 20 mM Tris base of 50ml solution?
To prepare a 20 mM Tris base solution of 50 mL, you would need to calculate the amount of Tris base needed using its molar mass and molarity formula (Molarity = moles of solute / liters of solution). Once calculated, weigh out the required amount of Tris base and dissolve it in deionized water to make a final volume of 50 mL. Mix thoroughly to ensure uniform distribution.
How do you prepare a 0.0001 M soulution HCL using 0.1 M of HCl?
you need to make 1000 times dilutions, this could be done in multi-steps: transfer 1 ml 0.1 M HCl into 100 ml volumetric flask and complete volume with water --------(1) from solution (1) transfer 2 ml into 20 ml volumetric falsk and complete volume with water, this is 0.0001 M HCL.
Calculate the mole fraction of HCl in 20 percent aqueous solution?
The mole fraction of HCl in a 20% aqueous solution can be calculated by converting the percentage to a molarity concentration. Assuming the density of the solution is 1 g/mL, a 20% solution means 20g of HCl in 100g of solution. If the molar mass of HCl is 36.5 g/mol, we can calculate the molarity and then use it to find the mole fraction of HCl in the solution.
How do you prepare 0.1M tris HCl buffer of pH 8?
Tris(hydroxymethyl)aminomethane (Tris) has a molecular weight of 121.14 g/mol. 50 mM = 0.050 mol/L (x 121.14 g/mol) = 6.057 g/L To prepare a 1L solution first weigh out 6.057 g Tris Add roughly 70% of final volume of water (i.e. 700 mL) Use a pH-meter to measure the pH of the solution Lower the pH of the solution to 7.2 using undiluted HCl Use a measuring cylinder or volumetric flask to make the volume up to 1000 mL If you add too much HCl you need to add more Tris and then recalculate the amount of water that you need add. In this case, every 1 g of Tris requires 165 mL of water to be added.
How do you convert 20mM tris-Hcl to gm?
let we have to calculate wait of 20mM tris-HCL for a solution of 1liter,then formula for molarity is Molarity= weight (in grams)/molecular weight X volume in liter hence 20/1000=wait/121.14X1 wait = 20 X 121.14/1000 (cross multiplication) wait = 2.42gm
What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?
1x PBS buffer typically has a molarity of around 0.01 M. To prepare a 20 mM PBS buffer, you would need to dilute the 1x PBS stock solution with water. For example, to make 1 liter of 20 mM PBS buffer, you would need to mix 2 ml of 1 M PBS stock solution with 98 ml of water.
How do you prepare 20 mM Tris base of 50ml solution?
To prepare a 20 mM Tris base solution of 50 mL, you would need to calculate the amount of Tris base needed using its molar mass and molarity formula (Molarity = moles of solute / liters of solution). Once calculated, weigh out the required amount of Tris base and dissolve it in deionized water to make a final volume of 50 mL. Mix thoroughly to ensure uniform distribution.
How do prepare 1X TE Buffer from 5X TE Buffer?
To prepare 1X TE buffer from 5X TE buffer, you would dilute the 5X TE buffer by mixing 1 part of the 5X buffer with 4 parts of water. For example, mix 1 ml of 5X TE buffer with 4 ml of water to obtain 5 ml of 1X TE buffer.
I have to prepare a 1 to 10 dilution and have a 2ml culture suspension and want to prepare a 1 to 10 dilution of the entire culture.?
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
How is purified genomic DNA stored?
Purified genomic DNA is typically stored in a buffer solution containing a stabilizing agent, such as Tris-EDTA (TE) buffer, to protect the DNA from degradation. Samples are usually kept at -20°C or -80°C to maintain stability and prevent enzymatic degradation. It is important to avoid repeated freeze-thaw cycles to preserve the integrity of the DNA.
What is the difference between tbs buffer-tbst buffer and corresponding function of them?
Tween 20. In TBST you add 0.05-0.1/ Tween 20.
How do you prepare a 0.0001 M soulution HCL using 0.1 M of HCl?
you need to make 1000 times dilutions, this could be done in multi-steps: transfer 1 ml 0.1 M HCl into 100 ml volumetric flask and complete volume with water --------(1) from solution (1) transfer 2 ml into 20 ml volumetric falsk and complete volume with water, this is 0.0001 M HCL.
Calculate the mole fraction of HCl in 20 percent aqueous solution?
The mole fraction of HCl in a 20% aqueous solution can be calculated by converting the percentage to a molarity concentration. Assuming the density of the solution is 1 g/mL, a 20% solution means 20g of HCl in 100g of solution. If the molar mass of HCl is 36.5 g/mol, we can calculate the molarity and then use it to find the mole fraction of HCl in the solution.
What is the buffer solution and why is it added to the agarose?
You use a buffer when making agarose gels so that when the gel is used for electrophoresis, the gel is able to conduct electricity. The buffer contains ions from the buffer salts that will facilitate conduction. that was good
How can we make 5 littre 30.5 percent Hcl from 33 percent and 20 percent Hcl?
Let A be the volume of 33% HCl and B the volume of 20% HCl Then, A + B = 5.........so, B = 5 - A And, 33A + 20B = 5 x 30.5 = 152.5 Substituting for B gives :- 33A + 20(5 - A) = 33A + 100 - 20A = 13A + 100 = 152.5 Thus, 13A = 152.5 - 100 = 52.5 : A = 52.5/13 = 4.0385 Litres (4dp) Which means that B = 5 - 4.0385 = 0.9615 Litres Mix 4.0385 Litres of 33% HCL with 0.9615 Litres of20% HCl to get 5 litres of 30.5% HCl.
