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How can you prepare borsches reagent in the lab?
To prepare Borsches reagent in the lab, mix equal volumes of concentrated sulfuric acid and glacial acetic acid in a fume hood. Then, slowly add 9 parts of water to the mixture while stirring constantly. Allow the reagent to cool before use.
What amino acids give positive Ehrlich test?
Amino acids that contain an indole group, such as tryptophan, give a positive Ehrlich test. The Ehrlich test is a colorimetric test that detects compounds containing indole or phenolic groups by producing a pink or red color when reacted with p-dimethylaminobenzaldehyde reagent.
How do you prepare Schultz Reagent?
To prepare Schultz reagent, mix one part of concentrated sulfuric acid with nine parts distilled water. Allow the mixture to cool before use. This reagent is commonly used for the detection of proteins in biological samples.
Why can't you prepare and store tollens' reagent in advance for tests?
Tollens' reagent is made by mixing two solutions - silver nitrate and ammonia - right before use because the reaction is sensitive to light, air, and temperature, which can degrade the reagent quickly. Preparing it in advance may lead to a loss of effectiveness, affecting the results of tests. Therefore, it is best to prepare Tollens' reagent fresh when needed for accurate testing.
What is the procedure to prepare anthrone reagent?
To prepare anthrone reagent, dissolve 200 mg of anthrone in 100 ml of concentrated sulfuric acid. Mix the solution thoroughly and allow it to cool before use. Anthrone reagent is used for the detection of carbohydrates, specifically sugars, in various biological samples.
What are the ingredients in Ehrlich's indole reagent?
Ehrlich's reagent, used to detect the presence of indole compounds, typically consists of p-dimethylaminobenzaldehyde dissolved in a strong acid, such as hydrochloric acid or sulfuric acid.
How can you prepare borsches reagent in the lab?
To prepare Borsches reagent in the lab, mix equal volumes of concentrated sulfuric acid and glacial acetic acid in a fume hood. Then, slowly add 9 parts of water to the mixture while stirring constantly. Allow the reagent to cool before use.
How top prepare liberman buchard spraying reagent for sterols?
liberman buchard spray reagent
What amino acids give positive Ehrlich test?
Amino acids that contain an indole group, such as tryptophan, give a positive Ehrlich test. The Ehrlich test is a colorimetric test that detects compounds containing indole or phenolic groups by producing a pink or red color when reacted with p-dimethylaminobenzaldehyde reagent.
How do you prepare millions reagent?
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How do you prepare Schultz Reagent?
To prepare Schultz reagent, mix one part of concentrated sulfuric acid with nine parts distilled water. Allow the mixture to cool before use. This reagent is commonly used for the detection of proteins in biological samples.
How do you prepare tollen reagent?
To prepare Tollens reagent, mix aqueous silver nitrate with ammonia solution until a precipitate forms. Then add sodium hydroxide solution to redissolve the precipitate and form the final reagent. It is used to test for the presence of aldehydes in a reaction.
How do you prepare nesslers reagent?
To prepare Nessler's reagent, dissolve 7g of mercuric iodide and 35g of potassium iodide in distilled water, and then add 100g of potassium hydroxide pellets. Once the solution is clear, dilute it with water to make 1 liter of Nessler's reagent. This reagent is commonly used for detecting ammonia in solutions.
How do you prepare seliwanoffs reagent?
To prepare Seliwanoff's reagent, dissolve resorcinol in concentrated hydrochloric acid, then add a small amount of acetaldehyde. Mix well and dilute with water if needed. This reagent is used for differentiating between aldose and ketose sugars based on the color developed upon heating.
How do you prepare kedd's reagent?
To prepare Kedde's reagent, mix equal volumes of concentrated hydrochloric acid and 5% potassium dichromate solution. The reagent is used to test for the presence of fatty acids in a substance, where a positive test results in the formation of a layer of orange crystals.
How do you prepare hager reagent?
To prepare Hager's reagent, mix equal volumes of 0.1 M silver nitrate (AgNO3) solution and 0.1 M ammonium thiocyanate (NH4SCN) solution in a clean glass container. This mixture will form a reddish-brown precipitate, indicating the formation of Hager's reagent.
Why can't you prepare and store tollens' reagent in advance for tests?
Tollens' reagent is made by mixing two solutions - silver nitrate and ammonia - right before use because the reaction is sensitive to light, air, and temperature, which can degrade the reagent quickly. Preparing it in advance may lead to a loss of effectiveness, affecting the results of tests. Therefore, it is best to prepare Tollens' reagent fresh when needed for accurate testing.
