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How does column chromatography separate a mixture of methylene blue and methyl orange?
Wiki User
∙ 15y ago
Methylene blue and methyl orange will have different binding affinities with the column material, and thus one will pass through the column more slowly than the other. This will result in one of the compounds being eluted from he column before the other. The one with the weakest binding to the column will be eluted first.
Wiki User
∙ 15y ago
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What is the importance of a mixed elution solvent in column chromatography?
In column chromatography, the stationary phase, a solid adsorbent, is placed in a vertical glass (usually) column and the mobile phase, a liquid, is added to the top and flows down through the column (by either gravity or external pressure). Column chromatography is generally used as a purification technique: it isolates desired compounds from a mixture.
Why does fluorenone travel in methylene chloride?
Presumably you are talking about in a chromatography column with alumina in it. The fluorenone is polar and subsequently creates a bond with the polar alumina. However, as Chlorine is more electronegative then the oxygens on alumina, the fluorenone wants to hang out and be friends with the methylene chloride even more than alumina, so the fluorenone and the methylene chloride hold hands and take a romantic walk down the column into your solution.
Why are the plates in column chromatography called 'theoretical plates'?
The number of theoretical plates in a chromatography column is a measure of how "long" the column is - how well it separates. A "short" column will only separate large or heavy molecules, and the medium and light stuff is still mixed together in the last band. A "long" column will separate the little stuff better because there are more theorectical plates. Picture a stack of sieves with smaller and smaller holes as the column gets "longer" and you've got the idea. This "length" has virtually nothing to do with the physical length of the separating column. It is a function of the packing materials and solvents used during a separation.
What is the retardation factor?
In chemical chromatography, it is a measure of the relative mobility of components of a mixture through a stationary phase while experiencing the forces of a mobile eluent phase, based on relative intermolecular attractive forces and molecular size. In thin layer chromatography, is it the ratio of distance travelled by a component compared to the distance travelled by the eluent front from the point of contact with the mixture. In column chromatography, it is the fraction of the component in the mobile phase at equilibrium. By comparison, in gas chromatography, relative retention times on the stationary phase are measured and compared for the mixture components.
What is sorbsil?
It is a column chromatography grade silica gel.
How do you separate the enzymes in column chromatography?
In column chromatography, the enzymes are made to pass through the column without occurrence of bubbles. These enzymes are obtained at the end of the process by slowly advancing through every column.
What is the importance of a mixed elution solvent in column chromatography?
In column chromatography, the stationary phase, a solid adsorbent, is placed in a vertical glass (usually) column and the mobile phase, a liquid, is added to the top and flows down through the column (by either gravity or external pressure). Column chromatography is generally used as a purification technique: it isolates desired compounds from a mixture.
Why do the pigments separate during column chromatography?
Because the retention coefficients of different substances are also different.
Why does fluorenone travel in methylene chloride?
Presumably you are talking about in a chromatography column with alumina in it. The fluorenone is polar and subsequently creates a bond with the polar alumina. However, as Chlorine is more electronegative then the oxygens on alumina, the fluorenone wants to hang out and be friends with the methylene chloride even more than alumina, so the fluorenone and the methylene chloride hold hands and take a romantic walk down the column into your solution.
What is the function of pump in HPLC?
A high-performance liquid chromatography, or HPLC, refers to a technique in analytic chemistry that is used to separate the components in a mixture. The pump in HPLC passes a pressurized liquid solvent that contains the sample mixture through a column filled with a solid adsorbent material.
What are the main applications of column chromatography?
Column chromatography is used in the lab and industry to isolate the compound that they want. Since some chemical reactions are not selective to the product you want, you have to get rid of the products you don't want. Sometimes column chromatography is the only way.
What technique is used to separate salt and sugar?
Any of the three types of chromatography (column , thin - layer or paper) can be used to separate the salt from sugar and vice-verse !
How can seprate p-nitro acetanilide mixture of para and ortho acetanilide?
The amide group on acetanilide is an ortho/para director, so a simple nitration should work: a mixture of sulfiric acid and nitric acid should be sufficient. Afterward, separation of the ortho and para compounds (by column chromatography, probably) would be necessary.
Searching for the sources where you can buy Silica Gel TLC Plates for Column Chromatography?
Column Chromatography is best choice for if you are looking for Silica Gel TLC Plates for Column Chromatography. For more inquiry call on 9879203377.
What is the difference between flash chromatography and column chromatography?
Column chromatography, is a broad term for all column chromatography methods, but is also synonomous with Gravity fed methods. Flash chromotography refers specifically to a column in which the eluant (or mobile phase) is moved through the column under pressure (using a hand pump for small scale, or a pressurised gas for a larger scale), the name Flash is derived from how much faster it is to run a column under pressure than via gravity.
What is the function of elution buffer in the DNA extration?
In column chromatography, it is put in the column to basically cleanse and lubricate. Generally, it helps to wash out any left-over proteins from a previous experiment. It can also help to separate the fractions that are collected.
How high-performace liquid chromatography works?
High-performance liquid chromatography (HPLC) works by pumping a liquid sample through a column packed with tiny particles. These particles have different affinities for the components of the sample, causing them to separate as they pass through the column. The separated components are then detected by a detector, which produces a chromatogram. HPLC is commonly used in analytical chemistry to separate and quantify compounds in a mixture.
