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I think it depends mostly on which formula you are using. The more commonly used formula for anion gap is [Na+] − ([Cl−] + [HCO3−]). If you are using the other equation that includes Potassium ([Na+] + [K+]) − ([Cl−] + [HCO3−]) remember that Potassium is significantly increased with hemolysis due to it being mainly intracellular. This would result in a false increase of the anion gap due to hemolysis. I don't think using the former equation would result in that significant of a change since the other electrolytes are not affected much by hemolysis.

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Q: How does gross hemolysis affect anion gap?
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