an electric current is passed through the agar in electroimmunodiffusion assays and therefore separates antigens based on their isoelectric point. Immunodiffusion does not involve electrophoresis and can be used to assess Ab to Ag binding through the visualization of a precipitate line
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Assay on 'as is' basis refers to the composition of a substance including its water content, while assay on 'dried' basis refers to the composition of a substance after removing the water content. Comparing these two types of assays can help in understanding the true chemical composition of a substance excluding the influence of water content.
xx.x% on a dried basis implies that the material will have had all possible liquid impurities (water, solvents, residual liquid chemicals) removed by the drying process.Let's call w% is % of (water content, solvents, and residual) all combine. Your as-is value would be:xx.x% * (100 - w%)% as-is = ---------------------100Hopes that help.
Potency is the purity of the compound ;this states % available of the main component without water and residual solvents Assay is defined as Potency plus water and residual solvents and other remaining fractions please note assay can also be expressed as as is basis or dry basis Assay and Potency are same if assay is exprtessed as as is basis please note Potency word is mainly used for Antibiotics and bilogicals courtsey ; CDER , USFDA ; Dr.Uttam Krishna
The optimal beta-mercaptoethanol molarity for maintaining protein stability in a biochemical assay is typically around 1-5 mM.
Pierre C. Arquembourg has written: 'Immunoelectrophoresis' -- subject(s): Immunoelectrophoresis 'Primer of immunoelectrophoresis' -- subject(s): Immunoelectrophoresis
The AGID test is the agar gel immunodiffusion test and ELISA is the enzyme-linked immunosorbent assay. Both test for the presence of certain proteins (called antigens) by binding them with antibodies.
the main reason to do immunodiffusion test is to quantify antigen or antibody
Immunoelectrophoresis-- 21 letters
SDS-PAGE method
Immunoelectrophoresis is performed by placing serum on a slide containing a gel designed specifically for the test. An electric current is then passed through the gel, and immunoglobulins, which contain an electric charge, migrate.
This test requires a blood sample.
a monoclonic increase of IgG globulin
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
The procedures for carrying out spectrophotometric and fluorometric GUS assays are similar in that they both involve measuring the enzymatic activity of the GUS enzyme, but they differ in the detection method. In the spectrophotometric assay, absorbance changes at a specific wavelength are measured, while the fluorometric assay measures fluorescence emitted from a substrate after the GUS enzyme acts on it. Additionally, the sensitivity and specific conditions for each assay may vary, influencing the choice of method based on the experimental requirements.
No, assay by mass balance and assay by as is basis are not equivalent. Assay by mass balance calculates the amount of a component based on material balance equations, while assay by as is basis measures the amount of a component without accounting for any changes or losses that may occur during processing.
Immunoelectrophoresis is a laboratory technique that combines electrophoresis and antibody-antigen interactions to separate and identify proteins in a sample. It is commonly used to analyze the composition of proteins in biological fluids, such as blood or urine, by exploiting the specific binding between antibodies and antigens.