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Applying too much sample on a TLC plate can lead to overcrowding of the compounds, causing them to run close together or even overlap. This can result in poor separation and difficulty in distinguishing different compounds. It may also lead to band broadening and decreased resolution, affecting the accuracy of the analysis.
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What is split ratio in gas chromato graphy?
The split ratio in gas chromatography refers to the ratio of the carrier gas that is split between the column and the detector. This ratio determines how much of the sample is directed into the column for separation and how much is directed to the detector for analysis. Adjusting the split ratio can affect the sensitivity, resolution, and detection limits of the analysis.
Why crystals disappear during suction filtration of a sample crystallized from benzene?
Crystals may disappear during suction filtration of a sample crystallized from benzene if the crystals dissolve in the solvent being used for filtration, or if they break up into smaller particles that pass through the filter. This can happen if the crystals are not fully dried or if too much force is applied during filtration.
Does sample size affect Density of a substance?
Not in normal circumstances. But if your sample contains only one atom, density becomes irrelevant, because practically all the mass is concentrated into the nucleus which is an object of extremely small size, much, much, much smaller than the size of the atom. Equally if the size becomes star-sized the gravity in some circumstances causes the very atoms to be crushed so that protons and electrons are crushed to form neutrons all packed tightly together and the density becomes literally astronomical ("neutron stars").
How do you determine which fertilizer to use?
Decide whether the crop to be grown will be organic or standard commercial.If organic, select manure (if autumn applied) or compost (if spring applied).If commercial, sample the soil in several locations of the field.Have soil sample tested.Follow fertility recommendation on report.If no soil sample is to be taken, analyze cropping history of field.Determine how much nutrients each crop has removed from the soil AND how much fertilizer has been added for each.Calculate fertility needs for new crop for each nutrient and apply same.
How do you calculate how much water is released in a sample of copper sulfate?
(CuSO4·xH2O) I would suggest heating in a crucible to a constant mass. A simple gravametric calculation using the balanced equation and m=n.gfm will give you a value for x x should be 5
Why is it important to use a small amount of sample when spotting a TLC sheet?
Spot small amount of sample so that it run as a discrete spot and doesn't diffuse out into a large area. It make the separation from other compounds much more efficient.
What will happen if too much heat is applied while heat fixing a slide?
Applying too much heat while heat fixing a slide can cause the sample to dry out too quickly, leading to distortion or loss of cellular structures. Additionally, excessive heat can cause the slide to crack or shatter, ruining the sample. It is important to use gentle heat when fixing slides to ensure optimal preservation of the sample.
Why Electrophoresis power supply results in longer gel run time?
There are several factors that control the rate at which a sample moves or migrates in a gel. One of those factors is electric power supply. The larger the voltage applied, the faster the migration. However, there is an upper limit to how much voltage can be applied. If the voltage is too high, it will cause heating in the electrophoresis module and this is turn will negatively affect the integrity of the gel.
How does the system of checks and balances affect the branches of government?
It allows them to make sure one branch does not have too much power, so that they all have an equal amount. This helps them with separation of the power.
What will be the result of applying too much compound to a TLC plate?
Applying too much compound to the TLC plate makes it difficult for a good separation to occur. Instead, a large blot of solution tends to appear in the TLC. "It is extremely important that the spots be as small as possible and that they be applied using a 1% solution of the compounds being separated
What is burst strength?
Bursting strength tells how much pressure paper can tolerate before rupture. It is important for bag paper.Bursting strength is measured as the maximum hydrostatic pressure required to rupture the sample by constantly increasing the pressure applied through a rubber diaphragm on 1.20 - inch diameter (30.5 mm) sample.
What is split ratio in gas chromato graphy?
The split ratio in gas chromatography refers to the ratio of the carrier gas that is split between the column and the detector. This ratio determines how much of the sample is directed into the column for separation and how much is directed to the detector for analysis. Adjusting the split ratio can affect the sensitivity, resolution, and detection limits of the analysis.
What property depends on the amount of matter in the sample?
Depends on what the sample is and how much of it you have.
How much work is done with a force of 300 N for 750 m if the force of friction is 200 N?
The idea here is to multiply the force applied - the 300 N - by the distance over which it is applied. The force of friction does not affect the calculation. Note: Since the force of friction is less than the force applied, and if there are no other forces, the object will accelerate.
How much of a 100 gram sample would remain unchanged after two hours?
This would depend on the specific sample and its stability. Without additional information, it is not possible to determine how much of the sample would remain unchanged after two hours.
Will an object move at a faster speed when force is applied to it?
yes depending how much you applied
Why crystals disappear during suction filtration of a sample crystallized from benzene?
Crystals may disappear during suction filtration of a sample crystallized from benzene if the crystals dissolve in the solvent being used for filtration, or if they break up into smaller particles that pass through the filter. This can happen if the crystals are not fully dried or if too much force is applied during filtration.
