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List two factors used to identify a substance by paper chromatography?
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If you're talking about the factors that affect movement of the substance (mobile phase) up the filter paper, that would be:
-size of particles
-solubility in the solvent
-adsorption to paper
Wiki User
∙ 13y ago
Wiki User
∙ 13y agoNot everything can be "separated" by paper chromotography. Assuming you know the setup for chromotography and the basic terminology; when you have two substance that have varying affinities for the mobile phase and the stationary phase, these two substances will have different, and distinct rf values. I but separate in quotes because you can't really separate the substances like this but in many cases you can tell how many different substances you have and what those substances are.
Wiki User
∙ 13y ago1- the solubility of the pigments
2- size of particles
3- attractiveness to the paper
Wiki User
∙ 10y agoSolubility and adsorption
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What is something that changes color in ways that lets you identify a substance?
Indicators such as litmus paper
How can litmus paper be used?
Litmus paper is simply used to check for acidity. It is not quantitative, only qualitative. That is, its use will not identify an exact pH, but its use can identify if the substance being checked is acidic or not.
When the amino acids are colourless how will you get to know that which acid is this?
It really depends on the type of chromatography. E.g. with thin layer chromatography, UV light is used, and the amount of light that is absorbed is measured, and they can tell the amino acid from this. In paper chromatography, a substance called ninhydrin is sprayed onto the separated amino acids and they become visible.
Why solvent is used in Paper chromatography?
In chromatography you need one stationery phase (paper) and one mobile phase (here solvent, moving by capillary forces)
What is eluent front?
Is the eluent on the front of the Chromatography paper.
What result do you get with paper chromatography to indicate a pure substance?
only contain one colour
Why is chromatography used in the police?
the police use chromatography to solve crimes, for example if they wanted to solve if a drug or a substance was illegal or legal they would use paper chromatograhy which would mean they would place the substance or a drug on paper dip it in a testing liquid which could be water for example and if the paper turns purple for example that liquid may be illegal or if that dot of the certain substance rises over 5cm for example it may be illegal so chromatography comes in very handy for the police and solving there crimes ! Hope this helped ;) xxx
What is the interpretation of paper chromatography?
whate is interpretation in paper chromatography
What is descending paper chromatography?
ascending chromatography is a type of chromatography in which chromatic substance is in mobile phase and moving from bottom to top. similarly in descending chromatography mobile phase moving from top to bottom.
What can you use instead of chromatography paper in a paper chromatography test?
newspaper
How is paper chromatography used in everyday life?
it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.
Does each spot on the developed chromatography paper or plate represent one substance?
That is correct. Every chemical substance travels at its own speed when diffusing through paper, and so every substance will wind up at its own location on the paper or plate as the case may be.
What is the result in candy chromatography?
The colors will bleed out onto the paper and you would be able to identify what and how many color dyes are in that candy piece.
Where may one learn about paper chromatography?
If you like science Paper Chromatography is something to look into. You can take Chemistry classes to find out different ideas on how to use Paper Chromatography.
What is chromatography paper called?
Whatman Paper
How does chromatography check the purity of a substance?
you put the blob off a blob off the substance you want to test on to a piece of chromotography paper about 10mm from the bottom then fill a flask about 20mm and let the bottom of the paper sit in the water if the substance is pure then there will only be one colour goes up the paper.
What is grass chromatography?
There is no such thing as grass chromatography. there is only High Performance Liquid Chromatography, Gas Chomatography, and Paper Chromatography.
