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what is the difference c3 and c4 hplc column?

Updated: 4/28/2022
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Rachael Donnelly

Lvl 10
2y ago

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The difference is the length of the carbon alkyl chain off of the silica bead support in the column. The higher the number, the longer the chain, the more "reverse phase" it is. For example a polar compound is best separated on a C3 if you must use reverse phase and a non-polar compound will be best separated on a C18 column.

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Daryl Kemmer

Lvl 13
2y ago
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Q: What is the difference c3 and c4 hplc column?
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