0
What advantages does hanging drop preparation have over a wet mount preparation?
Wiki User
∙ 15y ago
Hanging drop slide is more resistant to evaporation because of the concave depression of the slide placed over the cells along with the petroleum jelly prevents drying. Wet mounts can dry out fairly quickly.
Wiki User
∙ 12y ago
Wiki User
∙ 11y ago- Quick preparation: specimen fixation, dehydration and staining are not necessary (but possible, if required). For this reason, wet mounts are the first kind of mounts that students learn to make.
- Few artifacts: If there is no chemical and physical processing of the specimens before observation (no fixation), there are little artifacts and the specimens appear in their natural condition.
- Living and moving: It is possible to observe living and moving organisms. It is also possible to observe certain processes of life, such as feeding, cell division etc. (for water-based mounts)
- Natural colors: The colors are natural and not faded. The colors of permanently mounted specimens may fade over time.
Wiki User
∙ 15y agoTry to visit this site. :) http://www.microbiologyprocedure.com/microscopy-methods-in-microbiology/hanging-drop-preparation-and-its-modifications.html
Wiki User
∙ 15y agohe advantage of this preparation over the wet mount preparation is the increased capacity of aeration as the drop is surrounded by an air space.
Wiki User
∙ 14y agoPreservation of cell morphology, and you can see the little critters swim, if they're motile.
Wiki User
∙ 11y agoto see motility and method of movement
to see morphology
Add your answer:
Earn +20 pts
Why is petroleum jelly used in hanging drop method?
This type of sealing material is used primarily to reduce evaporation of liquid and to eliminate air current in the preparation.
What is the purpose of hanging drop technique?
The purpose of the hanging drop technique is a method in which a drop of bacterial suspension is enclosed in an air-tight chamber prepared in a special depression slide. It allows you to view living organisms.
What happens when a drop remains hanging from the tip of the burette after the solution has been added to the flask?
If there are any drops still hanging on the burette, they should be washed into the flask with distilled water.
What is the difference between wet mount and dry mount?
- a wet mount is freshly prepared while a dry mount is pre-prepared and sealed. - the specimen is alive in a wet mount while in a dry mount the specimen is dead - air bubbles can be trapped in a wet mount but not in a dry mount - wet mounts are priceless while dry mounts costs
How is a half drop titrant dispensed from a buret?
To dispense half a drop, you open the stopcork very carefuly and let the droplet appear and start to grow. Once the droplet reaches half it's usual size, close the stopcork. The droplet should now be hanging from the burette. Wash it down into the solution with your solvent wash bottle.
Aside from motility what facts learned from hanging drop preparation?
The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.
What specimen preparation is best for viewing cell motility?
hanging drop
What is the value of a hanging-drop preparation?
Hanging drop preparation is very valuable as it allows for a truer research capability. Hanging drop preparation offers longer observation abilities.
What is the Motion of pseudomonas fluorescens in hanging drop preparation?
Should be flagellar motion since it has falgella
Differentiate the motility of the microorganisms observed in wet mount from hanging-drop mount based on the rate of movement?
Movement of microorganisms from wet mount is slower than movement of microorganisms from hanging drop mount.
Would you expect Brownian motion to increase the longer you observe a hanging drop or wet mount preparation?
Just by the sheer fact that any vibration of any kind can be considered Brownian motion for a hanging drop test. the longer you observe your bacteria, the more chance there are of Brownian motion interfering with your motility test ( I assume you're talking about the motility hanging drop, right?). It is because of this fact that the hanging drop test is quickly falling out of favor for more secure tests for motility, the semi-solid media being one of them.
Motility is best seen with a?
Wet mount, hanging drop slide, or motility tube (SIMS, ONPG-PAM)
How stain is applied to a slide holding a wet mount preparation?
All you have to do is get a yellow stain and just drop one drop and it should spread throughout the water and should turn that color.
Why is petroleum jelly used in hanging drop method?
This type of sealing material is used primarily to reduce evaporation of liquid and to eliminate air current in the preparation.
Would you expect Brownian motion to increase the longer you observe a hanging drop preparation?
Just by the sheer fact that any vibration of any kind can be considered Brownian motion for a hanging drop test. the longer you observe your bacteria, the more chance there are of Brownian motion interfering with your motility test ( I assume you're talking about the motility hanging drop, right?). It is because of this fact that the hanging drop test is quickly falling out of favor for more secure tests for motility, the semi-solid media being one of them.
Does the hanging-drop technique have any practical value?
hanging drop tecnique have practical value
Does hanging drop technique have any practical value?
hanging drop tecnique have practical value
