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Some potential experimental errors that could affect hemoglobin (Hb) levels in Sahli's method for estimating Hb include inaccurate measurement of blood volume, improper mixing of blood with reagents, and variability in interpreting color changes due to human subjectivity. These errors can lead to inaccurate readings and affect the reliability of the Hb level determination. It is important to follow the protocol carefully to minimize such errors.
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Can dilute nitric or sulphuric acid be used instead of hydrochloric acid in sahlis acid hematin method?
Yes, diluted nitric or sulfuric acid can be used instead of hydrochloric acid in Sahli's acid hematin method. However, the choice of acid may affect the sensitivity and accuracy of the results, so adjustments may be needed in the protocol or interpretation of the test. It is advisable to validate the method with the alternative acid before routine use.
Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
Why lower meniscus considered in sahlis method?
In Sahli's method, the lower meniscus is considered because it is more accurate to take readings at the bottom of the meniscus where the liquid level is the most stable. This helps to ensure precise measurement of hemoglobin concentration in the blood sample.
Can dilute nitric or sulphuric acid be used instead of hydrochloric acid in sahlis acid hematin method?
Yes, diluted nitric or sulfuric acid can be used instead of hydrochloric acid in Sahli's acid hematin method. However, the choice of acid may affect the sensitivity and accuracy of the results, so adjustments may be needed in the protocol or interpretation of the test. It is advisable to validate the method with the alternative acid before routine use.
Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
