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13y ago

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Why do Forensic scientists sometimes use chromatography to investigate paints left at a crime scene and what could this tell them?

Forensic scientists use chromatography to separate the components of paint samples, which can reveal the chemical composition of the paint. This information can help match paint from a crime scene to a particular source, such as a suspect's car, or to determine if different paint samples originated from the same source.


What are the avantages of thin layer chromatography over paper chromatography?

Thin layer chromatography typically provides better resolution and separation of compounds due to the use of a uniform, inert stationary phase. It also offers faster separation times and requires smaller sample volumes compared to paper chromatography. Additionally, thin layer chromatography allows for visualization of separated compounds under UV light without the need for chemical staining.


What is the separation of mixtures based on?

The physical properties of the things in the mixture. It could be that one part of the mixture is soluble, the other part insoluble - both physical properties. It could be that you use the boiling point of 2 liquids to separate them - physical properties. You could use a magnet if one part is magnetic and the other isn't - physical properties. And so on. But you are relying on physical properties rather than chemical properties.


What technique would you use to separate calcium nitrate and sodium acetate?

One method to separate calcium nitrate and sodium acetate is through precipitation. By adding a solution that forms a precipitate with one of the compounds but not the other, you can then separate the two components based on their solubility differences. Alternatively, you could also consider using techniques such as crystallization or chromatography for separation.


When the amino acids are colourless how will you get to know that which acid is this?

It really depends on the type of chromatography. E.g. with thin layer chromatography, UV light is used, and the amount of light that is absorbed is measured, and they can tell the amino acid from this. In paper chromatography, a substance called ninhydrin is sprayed onto the separated amino acids and they become visible.