What are roles performed by sodium sulfide and ethanol in catalase and amylase?

Answer 1

Sodium sulfide is often used as a reducing agent in biochemical reactions involving enzymes like catalase and amylase. It helps to maintain the enzyme's active conformation by preventing the formation of disulfide bonds that could disrupt its structure. Ethanol, on the other hand, is commonly used as a denaturant to disrupt enzyme activity by altering the enzyme's tertiary structure. In the context of catalase and amylase, ethanol can be used to inhibit or deactivate the enzymes by disrupting their active sites.

Answer 2

Sodium sulfide is a reducing agent that helps in deactivating catalase enzyme by reducing the active site's heme iron. Ethanol can denature both catalase and amylase enzymes by disrupting their structure and altering the active sites, leading to loss of enzyme activity.

Answer 3

Sodium sulfide and ethanol in catalase and amylase can speed up the the enzyme in some starch.