Rf Values determine the solubility of a substance with respect to a certain solvent.
It also determines the affinity of the solute to the solvent
(greater Rf=greater affinity of solute to the solvent)
In column chromatography, the retention factor is defined as the ratio of time an analyte is retained in the stationary phase to the time it is retained in the mobile phase
the difference between retention time and retention factor is that the retention time is a quatitative value where`s the retention factor is a qualitative on.
Retention Factor Rf == Distance traveled / total distance
Using isocratic retention parameters, the gradient elution retention time for several proteins has been calculated. The gradient retention time calculation is based on fitting the isocratic retention data to an equation of the form: log k′ = m log (1/[Ca2+]) + log K and on applying well-established principles of gradient elution. A good correlation between the observed and calculated retention times for several test proteins was obtained at various total gradient times and column flow-rates.Conversely, isocratic retention parameters characterizing protein retention can be calculated from gradient elution retention data. However, even with retention data of high quality, small errors are amplified by the log-log nature of the ion-exchange isocratic retention model employed.Based on the close correlation between predicted and observed gradient retention times, no evidence for protein denaturation resulting from immobilization of the protein at high initial k′ values at or near the column inlet was observed.
Rf= distance travelled by pigment (cm)/distance travlled by solvent (cm)
Retention time decrease with an increase in pH.
tlc
the difference between retention time and retention factor is that the retention time is a quatitative value where`s the retention factor is a qualitative on.
What is the significance of negative values of voltage and current?Negative values show direction and that is the significance
No
Hygroscopic
it depends on the retention time, void time and reagents that you were used on your chromatograph.
The retention factor of a particular material is the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin.
Selective retention, in relating to the mind, is the process when people more accurately remember messages that are closer to their interests, values and beliefs.
Rf Values determine the solubility of a substance with respect to a certain solvent.
There is no significance to a power factor of 0.82.
Retention Factor Rf == Distance traveled / total distance
Axiology