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In the Salkowski test, the upper chloroform layer develops a yellow color due to the reaction between tryptophan and sulfuric acid. This color change indicates the presence of indole compounds, which are produced by some bacteria during tryptophan metabolism.

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1y ago

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If chloroform dilute in an organic solvent will it form the upper layer or the lower layer?

Chloroform is less dense than most organic solvents and will usually form the upper layer when mixed with them. This is due to its lower density and immiscibility with many organic solvents, causing it to float on top.


Water and chloroform are mixedthe components separate into two layerseach of which smells strongly of chloroformHow would you quickly and easily confirm which layer is water and which is chloroform?

You can quickly confirm which layer is water and which is chloroform by adding a few drops of water to a test tube containing the layers. Water will mix with the layer that is water, causing it to become more transparent. Chloroform will not mix with water and remain as a separate layer.


What is the positive result and the importance of liebermann burchard and salkowski tests?

it is a test for presence of cholestrol and if two layers form, upper layer becomes red and lower one becomes yellow, it indicates the presence of cholestrol.


Explain briefly how you would separate chloroform or dichloromethane extract from an aqueous solution using a separating funnel?

To separate chloroform or dichloromethane extract from an aqueous solution using a separating funnel, you would add the mixture into the funnel and allow the layers to separate based on their densities. Then, carefully drain the lower aqueous layer while keeping the organic layer in the funnel. Finally, collect the organic layer in a separate container and repeat the process if needed for further purification.


How the upper layer of the atmosphere differ from bottom layer?

The upper layer is different from the bottom layer because the bottom layer has more gualities to a living thing than the upper layer.


How the upper layers of the atmosphere differ from the bottom layer?

The upper layer is different from the bottom layer because the bottom layer has more gualities to a living thing than the upper layer.


What is upper layer made of?

upper layer is made of mantle of the earth.


In the upper layer of the atmosphare ozone is formed by?

The upper layer of the atmosphere is formed by ozone layer. This is a layer of ozone gas.


What does the layer in the upper atmosphere do?

The layer in upper atmosphere is ozone layer. It protects us from UV rays of the sun.


Do you observe the halide test in the upper layer or the lower layer?

upper layer


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What kind of burn develops blisters and looks wet on the skin?

This is a second degree burn, as it has blistered. This means more of the skin was affected than the epidermis, or upper most layer of skin.