reverse transcriptase
It depends if you mean Reverse transcription PCR (RT-PCR) or real time PCR (qPCR) - (there are misnomers often used with real time being (RT)Earliest article I could find on reverse transcription isCoupled reverse transcription-polymerase chain reaction (RT-PCR) as a sensitive and rapid method for isozyme genotyping. Gene, 1990, 93, 271-275 published by Mocharla, H., Mocharla, R., Hodes, M. E.,
prohibitors are used to enzyme reaction
Transcription is located in the nucleus, and translation is located in the cytoplasm. The product of transcription is RNA, and the product of translation is protein. The reactants for transcription is DNA, and the reactants for translation is mRNA and tRNA.
When the Operation is replication these enzymes are the Dna Replicases; when the Operation is Transcription these enzymes are the Rna synthetases.
The nucleotides that are involved in transcription are known as uracil (U). This is what will be used in the RNA transcription to the ribosomes.
Rna reverse transcriptase.
Reverse transcription is a process where a reverse transcriptase enzyme is used to generate complementary DNA from an RNA template. It is needed for the replication of retroviruses.
The reverse transcriptase enzyme is responsible for synthesis of DNA from an RNA template by the process of reverse transcription.
The process that is used to create DNA from an RNA template is called reverse transcription (commonly found in retroviral infection). The enzyme that aids in this process is called reverse transcriptase.
In normal transcription DNA is converted to RNA .reverse transcription is nothing but RNA will be converted to DNA
Retroviruses are considered a special class of viruses because they transcribe RNA to DNA using reverse transcriptase. HIV uses reverse transcription to synthesize a DNA strand using its RNA genome as a template.
RNA polymerase.
The enzyme, reverse transcriptase, uses an RNA template to create DNA. Then transcription is done normally to recreate an RNA template and then have it expressed as a polypeptide. By adding this extra step, the chance of mutations is greater so this process is important for quicker adaptations. For example, HIV uses reverse transcriptase and carries RNA instead of DNA.
Reverse transcriptase is the enzyme used to synthesize DNA from mRNA.
You might be referring to the reverse-transcription that many retroviruses use (a virus with an RNA that turns into DNA). By using the enzyme reverse transcriptase, viruses like HIV can make a DNA from RNA template.
When RNA's base sequence is used to determine the base sequence of a new strand of DNA, that is called reverse transcription.This is because the process is the reverse of transcription, which involves copying the base sequence of DNA to form RNA, including messenger RNA (mRNA).
RNA Polymerase