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A cuvette is, basically, a glorified test tube for use in a spectrometer. They're sometimes square in cross section to avoid refraction artifacts. Depending on what part of the spectrum is under consideration, they may be made of quartz, though plastic and glass cuvettes do exist.
You put a liquid sample into it then put the cuvette into the instrument. The beams inside of the instrument can 'see through' the quartz and 'read' your sample.
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∙ 11y ago
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Why is the line on the cuvette always aligned with that of the sampler holder?
This technique prevents scratching of the cuvette in those areas through which the light will pass. Scratches on the cuvette can lead to erroneous measurements.
What happens if you use a 0.5cm cuvette in a 1cm cuvette spectrophotometer?
The absorption of sample at maximum wavelength will be reduced by1/2 . Vijay Bhoir (Flamingo pharmaceuticals Ltd.Rabale Navi Mumbai ).
Why will acetone ruin a plastic cuvette?
Acetone corrodes some plastics.
If your cuvette had been dirty how would this have affected the value of ksp?
It will decrease the Ksp
Why do you use quartz cuvette rather than using glass cuvettes?
Glass will absorb radiation below 320nm whereas quartz will allow transmission of the UV wavelengths. So use glass cuvettes for wavelengths in the visible range from 380nm to 780nm and quartz cuvettes for wavelengths below 380nm.
Identify the functions of each of the cuvettes Cuvette 1?
1: control, recalibirate spectrophotometer 2: photosynthesis does not occur without light 3: photosynthesis does occur with light and live chloroplasts 4: boiling chloroplasts=no photosynthesis 5. control
Why is the line on the cuvette always aligned with that of the sampler holder?
This technique prevents scratching of the cuvette in those areas through which the light will pass. Scratches on the cuvette can lead to erroneous measurements.
What is miska in Russian?
A bowl. A cuvette.
Whats a little baby test tube called?
Cuvette
What happens if you use a 0.5cm cuvette in a 1cm cuvette spectrophotometer?
The absorption of sample at maximum wavelength will be reduced by1/2 . Vijay Bhoir (Flamingo pharmaceuticals Ltd.Rabale Navi Mumbai ).
Why Cuvette is used rectangular shape not spherical?
The rectangular shape avoids the curvature of light where as the spherical shape will bend it and give an inaccurate reading of absorption.
Why will acetone ruin a plastic cuvette?
Acetone corrodes some plastics.
If Cuvette 5 transmittance changed what is concluded?
27? lol 24!
How is your blank cuvette different from your other cuvettes?
It is your control. Your baseline reading.
What happens to results when the cuvette is not correctly placed?
You get no light transmittance or absorbance.
Where could one buy a cuvette online?
There are many online sites where one could go and purchase a cuvette online. These online sites include Amazon, Starnacells, Bio-Rad, and many more sites.
What was the purpose of adding three drops of chloroplast suspension to Cuvette 1?
k
