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What is alkaline reversion?

Updated: 4/28/2022
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15y ago

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Alkaline reversion is a condition in which the medium turns yellow during the first part of the incubation period and then changes to red later due to increased alkalinity by ammonia produced as proteins degrade.

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Q: What is alkaline reversion?
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How do you avoid alkaline reversion?

Alkaline reversion happens when microbes metabolize all the sugar with production of acid, then with continued incubation, the amino acids in peptones are metabolized and ammonia may be released as a waste. this in turn reacts with water in the medium to form the base ammonium hydroxide, an alkaline waste, which neutralizes the acid and elevates the pH So the way to avoid alkaline reversion is to elliminate the microbe concerned.


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