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Is Agar a homopolysaccaride or heteropolysaccaride?
Agar is a heteropolysaccharide composed of agarose and agaropectin. Agarose makes up the majority of agar and consists of repeating units of agarobiose, while agaropectin is a minor component with a more complex structure.
Seaweed derivative used to solidify microbiological media is?
One of the more common gelling agents used in microbiology is Agar, or more specifically agarose and agaropectin, polysaccharides derived from red algae (of which several species are commonly referred to as "seaweed").
What is the source and the chemical nature of agar?
Commercial agar is harvested from the cell walls of the Red Algae- Gelidium amansii. Agarose is a linear polymer that consists of alternating residues of D-galactose and 3,6-anhydro-L-galactose.
Why do most microorganisms fail to use agar as a carbon source?
Because the agar remains a solid anywhere below 42 degrees Celsius. A typical environment is never going to raise above 42 degrees therefore it will always remain solid even in the human body (37 degrees Celsius). Having that said, most microorganisms cannot digest agar unless it is liquid.
What is the chemical composition of agar?
chemically, agar is a galactan, a complex carbohydrate with galactose molecules which are not metabolized by non-ocean microbes. *** It has been know that only marine bacteria can degrade agar, but there are some reports that some soil bacteria can degrade and utilize the agar. http://www.jstage.jst.go.jp/article/bbb/67/5/67_1048/_article/-char/en
What is differences between agar and agarose?
Agarose is made from agarose, a polysaccharide from see weeds. Polyacrylamide is made from the synthetic polymerization of acrylamide, which in its monomeric form is a neurotoxin. Based on these structural differences, it could be said that agarose gels have larger 'pores' than polyacrylamide gels meaning that large particles can move more easily in agarose gels since the agarose polymers are larger and pack less densely then an equivalent amount of polyacrylamide. Therefore, agarose is generally used for the electrophoresis of large molecules such as DNA and RNA or speedy separation (low resolution) of small molecules such as proteins. Polyacrylamide is used for the high resolution electrophoresis of small molecules such as proteins.
