effect of solvent on UV-Visible spectrum
the solvent must have high transmittance, i.e, low absorbance.
effect of solvent on UV-Visible spectrum
Yes
Spectronic 20 was also an UV-Vis spectrophotometer.
khasm twada
Because nonmetallic elements are in vacuum UV
Because Only holmium can give sharp peaks in both UV and Vis range, Sharp peak is very rare in UV/Vis spectroscopy. It is very stable and doesn't absorb light at lower wavelength.
actually captopril is a PH sensative drug, as per IP-in 0.1N HCL it's showing 212nm, and it's very difficult to find out lambda by UV-spectroscopy because the UV visible range of UV- is 200-400nm, and generally near to 200nm all lambda consider as solvent pick....so UV-spectoscopy is not perfact one for analysis purpose of captopril.
Mass spectrometry, UV/Vis spectroscopy, NMR spectroscopy CNMR spectroscopy, Infra red spectroscopy
1 infra-red (UV-VIS) spectroscopy. 2 proton magnetic resonance spectroscopy. 3 carbon 13 magnetic resonoce spectroscopy.
Yes
By the Huckel determinant
Spectronic 20 was also an UV-Vis spectrophotometer.
khasm twada
Woodward Fieser rules are a set of emperical rules to calculate lambda max. in UV spectroscopy theoretically. They can be used to calculate the wavelength of maximum absorption of dienes and conjugated carbonyl compounds.
A wavelength vs absorbance graph depicts in uv spectroscopy shows the different colored wavelenths of UV light and how they are absorbed and percieved, and which ones are visible and which ones are not.
Each compound has a specific absorption spectra.
Because nonmetallic elements are in vacuum UV
you can determine the size of nanoparticles from UV-VIS spectroscopy. it is also comparable with TEM analysis.with below formula you can determin ethe size of nanoaprticles. d = Ln(landa SPR- landa0)/L1/L2