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the mixture turn purple. cuso4 n naoh is use to detect the presence of peptide bonds in protein

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How can you test for the presence of proteins?

Biuret Test for presence of proteins:Principle:The test indicates the presence of peptide linkages(CO-NH) in proteins. The CO-NH groups in polypeptide chain form complex colour compounds with cupric hydroxide (Cu(OH)2) which is formed by the action of NaOH on CuSO4 .Reagents:1) 40% Sodium hydroxide(NaOH)2) 1% Copper sulphate(CuSO4)Procedure:In 3mL of sample solution add 3mL of 40% NaOH. To This mixture add few drops of 1% CuSO4 .Observation:Purple Violet or Pink colour develops.Inference:(CO-NH) linkages are present.


What is general test for protein?

1. Add 5 cm3 dilute sodium hydroxide (caustic!) to a 1% solution of albumen (the white part of an egg)2. Then add 1% copper sulphate solution3. A purple colour appears and this indicates that protein is present.


How can I find out whether a unknown mixture has protein in it?

The most common method is the biuret test: in the presence of a copper(II) solution and NaOH the sample become violet.


Detect peptide bond?

A peptide bond is a covalent bond formed between the carboxyl group of one amino acid and the amino group of another amino acid during protein synthesis. It results in the formation of a dipeptide and releases a molecule of water. Peptide bonds are crucial for linking amino acids together to form polypeptide chains in proteins.


What is the complex formed between Cu2 plus and protein in biuret test?

In the biuret test, the complex formed between Cu2+ and protein is a coordination complex where the peptide bonds in the protein act as ligands to chelate with the Cu2+ ions. This complexation causes a color change, typically from blue to violet or purple, indicating the presence of proteins in the sample.


What will happen if heat crystals of CuSO4 pentahydrate in a test tube and note the water given off allow to cool and compare the color of anhydrous CuSO4 with original crystals?

Heating crystals of CuSO4 pentahydrate in a test tube will cause the water molecules trapped in the crystal lattice to evaporate, leaving behind anhydrous CuSO4 crystals. The color change observed will be from blue (for the hydrated form) to white (for the anhydrous form).


What substance is used as a control in a Biuret test?

Biuret reagent is made up of sodium hydroxide and copper sulphate.


What is the test to conclusively identify zinc ions a solution?

NaOH


What is the function of acidified cuso4?

Acidified CuSO4 is used as a reagent in the Fehling's test to detect the presence of reducing sugars in a solution. When a reducing sugar is present, it reduces the blue Cu2+ ions in the CuSO4 solution to red-brown Cu2O precipitate, indicating a positive result.


What lab tests are used to test for the presence of protein?

Common lab tests used to test for the presence of protein include a serum protein electrophoresis (SPEP) test, a urine protein test, and a total protein test. These tests can help diagnose conditions such as kidney disease, liver disease, or protein deficiencies.


How can one test for protein in a sample?

One can test for protein in a sample by using a method called the Biuret test. This test involves adding a reagent to the sample, which causes a color change if protein is present. The intensity of the color change can indicate the amount of protein in the sample.


What does T Protein indicate on a blood test?

T protein = Total protein