In reference to the melting of DNA: as DNA melts (denatures from a double-stranded molecule to two single strands) the UV absorbance INCREASES. This absorbance increase is referred to as a "hyperchromic shift" or the hyperchromic effect. Thinking about this situation in reverse: the UV absorbance DECREASES as two DNA strands anneal to form double stranded DNA. This is referred to as the "hypochromic effect".
(Please note, there is an answer on answers.com that incorrectly states the opposite, that absorbance decreases with melting. This is incorrect. Two single strands of DNA have higher absorbance than the double-stranded molecule.)
The effect of auxochrome on a chromophore is described by terms like bathochromic shift (red-shift), hypsochromic shift (blue-shift), and hyperchromic effect (increase in absorbance intensity). These terms refer to the changes in the absorption spectrum of a molecule due to the presence of an auxochrome group.
Hyperechromic shift refers to an increase in staining intensity or color, often seen in abnormal cells with increased DNA content. Hypochromic shift, on the other hand, refers to a decrease in staining intensity, typically seen in cells with reduced hemoglobin content, such as in certain types of anemia.
To effect something is to change it. It can also be something that is done in a movie or play (a special effect). An example would be: "The effects of the flies on the dogs was hilarious".
When something happens as a result of a cause, it's known as an "effect." The effect is the outcome or consequence of the cause.
An alternative synonym for the word "effect" is "outcome" or "result."
* Cell color (blood cells): (generally refers to the staining characteristics which reflects the hemoglobin concentration. Terms that describe hemoglobin content end with "chromic".) ~ normochromic (sufficient or normal amounts of hemoglobin) ~ hyperchromic (containing an unusually high concentration of hemoglobin in its cytoplasm) ~ hypochromic (containing an abnormally low concentration of hemoglobin)
The effect of auxochrome on a chromophore is described by terms like bathochromic shift (red-shift), hypsochromic shift (blue-shift), and hyperchromic effect (increase in absorbance intensity). These terms refer to the changes in the absorption spectrum of a molecule due to the presence of an auxochrome group.
A less intense hyperchromic shift indicates a smaller increase in DNA absorbance, suggesting a reduced DNA concentration or increase in single-stranded DNA compared to double-stranded DNA. This shift can result from factors such as DNA degradation, RNA contamination, or incomplete denaturation of DNA during spectrophotometric analysis. Further investigation may be needed to determine the specific cause of the reduced hyperchromic shift.
Iron-deficiency anemia
microcytic hypochromic anaemia
Microcytic hypochromic anemia is a condition where the red blood cell count and hemoglobin levels are low (hence anemia), with small red blood cells (microcytic), and red blood cells that stain less intensely than normal (hypochromic). The most common cause is iron deficiency. Low levels of iron in the body.
Hypochromic refers to red blood cells that have a reduced amount of hemoglobin, resulting in a paler color. This can indicate anemia, a condition where the body does not have enough red blood cells or hemoglobin to carry sufficient oxygen to tissues.
Hyperchromic anemia can cause a lower red blood cell count and higher levels of hemoglobin in the red blood cells. Other effects include brittle nails, sore mouth, headaches, and shortness of breath.
Microcytic hypochromic anemia is a condition where the red blood cell count and hemoglobin levels are low (hence anemia), with small red blood cells (microcytic), and red blood cells that stain less intensely than normal (hypochromic). The most common cause is iron deficiency. Low levels of iron in the body.
Hypochromic shift is observed in UV VISIBLE spectroscopy. This is the shift where the intensity of the absorption maxima is decreased
Hyperechromic shift refers to an increase in staining intensity or color, often seen in abnormal cells with increased DNA content. Hypochromic shift, on the other hand, refers to a decrease in staining intensity, typically seen in cells with reduced hemoglobin content, such as in certain types of anemia.
Lupus erythematosus systemicus