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In reference to the melting of DNA: as DNA melts (denatures from a double-stranded molecule to two single strands) the UV absorbance INCREASES. This absorbance increase is referred to as a "hyperchromic shift" or the hyperchromic effect. Thinking about this situation in reverse: the UV absorbance DECREASES as two DNA strands anneal to form double stranded DNA. This is referred to as the "hypochromic effect".

(Please note, there is an answer on answers.com that incorrectly states the opposite, that absorbance decreases with melting. This is incorrect. Two single strands of DNA have higher absorbance than the double-stranded molecule.)

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