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Luminol is a relatively simple chemical containing only carbon, nitrogen, oxygen and hydrogen and is a white to slightly yellow crystalline solid powder, soluble in water and most polar organic solvents.

Luminol is used by forensic investigators to detect trace amounts of blood left at crime scenes. Although it can detect small traces of blood, forensic investigators use this only as a last resort because it also reacts with soils, detergents, bleaches, carpets, metal objects, tools, plastic panels, wood, and some vegetable compounds.

To exhibit its luminescence, the luminol must first be activated with an oxidant. Usually, a solution of hydrogen peroxide (H2O2) and a hydroxide salt in water is used as the activator. In the presence of a catalyst such as an iron compound, the hydrogen peroxide is decomposed to form oxygen and water:

2 H2O2 → O2 + 2 H2O

In a laboratory setting, the catalyst used is often potassium ferricyanide. In the forensic detection of blood, the catalyst is the iron present in hemoglobin. Enzymes in a variety of biological systems may also catalyze the decomposition of hydrogen peroxide.

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Who discovered luminol?

Luminol was discovered by German chemist Heinrich Caro in 1908.


The forensic technician at a crime scene has just prepared a luminol stock solution by adding 17.0 of luminol into a total volume of 75.0 of?

The concentration of the luminol stock solution is approximately 22.7 M.


How does temperature affect luminol at a crime scene?

If the temperature is cold then the luminol will have a brighter excited state than if the temperature was hot or room temp.


What is luminol used for in crime scenes?

The basic idea of luminol is to reveal blood traces with a light producing chemical reaction between several chemicals and hemoglobin.


What reacts with the blood to cause luminesce?

Luminol


Explain why ivestigators may spray a crime scene with HCI before using luminol?

Investigators may spray a crime scene with hydrochloric acid (HCl) before using luminol to help remove any potential contaminants or residues that could interfere with the reaction of luminol with blood. This ensures a clean surface for the luminol to detect any hidden blood traces effectively.


Why does the luminol light reaction stop after a period of time?

The luminol reaction is an example of "chemiluminescence". This is when a chemical reaction yields a product in an electronically excited state (at least one electron in the product is at a level above the ground state). The excited product loses energy in the form of a photon of light when the electron drops to the ground state level. In the case of luminol the reaction is an oxidation under alkaline conditions and the reaction stops when either all the luminol, all the oxidising agent or all the alkali has been converted


What is an episode from CSI that uses luminol?

In Season 6, Episode 7 of CSI: Crime Scene Investigation titled "A Bullet Runs Through It, Part 1," luminol is used to detect blood in a crime scene. The forensic team sprays luminol on a crime scene and discovers blood spatter patterns that help them piece together what happened.


How is luminol made?

Luminol is synthesized from hydrazine and phthalic anhydride. The reaction involves several steps, including oxidation and cyclization, to form the luminol molecule. The final product is a chemiluminescent compound that emits light when it reacts with an oxidizing agent, making it useful in forensic investigations.


What other chemicals besides iron and copper can act as catalysts for the luminol reaction?

Other chemicals that can act as catalysts for the luminol reaction include hemoglobin and horseradish peroxidase. These catalysts can enhance the chemiluminescence produced when luminol reacts with hydrogen peroxide in the presence of an appropriate metal ion.


Does the glow of luminol last longer?

The glow of luminol does not last long, typically a few seconds to a minute. After the chemical reaction occurs and the luminescence fades, the glow will not be visible.


How do you make homemade luminol?

It is much more economical to purchase Luminol from a scientific supply store than to prepare it . The ingredients to make it are expensive and some of them are very difficult to obtain. When handling Luminol wear gloves and protective eye cover. It can cause inflammation to bare skin and eyes. Be sure to follow the manufacturers instruction for use.