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The TPTZ-Fe reaction in antioxidants tests the ability of various fruits, nuts, and food items to reduce Fe 3+ to Fe 2+ which binds TPTZ (2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ) very tightly with an extinction coefficient equal to 21,600 at 593 nm. The binding of Fe 2+ to this ligand creates a very intense navy blue color and this absorbance can be measured to test the amount of iron reduced.

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Q: What is the TPTZ -Fe Reaction in antioxidant power?
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