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The amount of oxygen taken up by the tissues in a muscle. It is the difference between oxygen concentration in the arteries and the oxygen concentration in the veins.
ie. if your blood carries 20ml of oxygen per 100ml of blood and the muscle takes up 6mls of oxygen, 14mls of oxygen leaves the muscles into the veins.
therefore, VO2-diff = 20ml-6ml = 14ml.

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