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What is the advantages of using biuret reaction in place of measuring the absorption of the protein solution at 280 nm?
Wiki User
∙ 16y ago
The absorption of proteins at 280nm is according to electrons from the rings on the amino acid such as His, Trp, etc. And if there is no such kind of amino acids in the protein, we might not be able to get what the amount of the protein really is. At the other hand, what makes biuret reaction work is by the copper ion reacting with the dipeptide bonds, since every amino acid has the peptide bond, it's more accurate and reasonable to use biuret reactions to determine what the amount of the protein is.
Wiki User
∙ 16y ago
Wiki User
∙ 11y agoHigh advantage of using Biuret assay is obtaining high accuracy results. The Biuret assay is rich enough to abstained proteins in concentrations between 0.005 and 2 mg/mL thus it refer as "Pierce assay" after the manufacturer of a reagent kit.
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What is the difference between absorption and stripping?
An absorption is a separation process where a gas is absorbed to a liquid. For example, an aqueous gas that has a tendency to leave solution can be forced to stay in solution through the addition of an absorbent. Stripping is the reverse separation reaction. A stripping process is the removal of solute from a liquid phase to a gas phase.
When you determine the pH of a solution what are you measuring?
The acidity of a solution. It can either be acidic, or basic.
What is the calibration line in a spectrophotometer used for?
To determine the concentration of a solution. Since the absorption of that solution is directly proportional to the concentration, the concentration of the solution can be determined.
When an exothermic reaction occurs in a water solution what happens to the temperature of the solution?
The temperature rises. An "exothermic" reaction releases heat energy in the process.
What does a precipitation reaction is a reaction between two aqueous solutions that results in the formation of a compound?
solutions
What is the difference between absorption and stripping?
An absorption is a separation process where a gas is absorbed to a liquid. For example, an aqueous gas that has a tendency to leave solution can be forced to stay in solution through the addition of an absorbent. Stripping is the reverse separation reaction. A stripping process is the removal of solute from a liquid phase to a gas phase.
In which unit absorption of the solution measured in?
liters
How do you monitor the rate of reaction when lumps of calcium carbonate dissolve in dilute hydrochloric acid solution?
by measuring the amout of gas produced, divided by the time taken
Pictures and uses of laboratory equipments?
Beaker - measuring volume of the solution Measuring cylinder - measuring volume
When you determine the pH of a solution what are you measuring?
The acidity of a solution. It can either be acidic, or basic.
What is silar method?
SILAR - SUCCESSIVE IONIC LAYER ABSORPTION AND REACTION is a low temperature and solution based process that can deposit atomic layers one layer at a time. The traditional SILAR cycle for a substrate is Dip in Cation precursor solution Rinse with distilled water Dip in Anion solution Rinse with distilled water
What is ex thermal reaction?
An Exothermal reaction is a chemical reaction that produces heat making the solution warm or hot. It is the opposite of an Endothemic reaction which makes the solution colder.
What is the calibration line in a spectrophotometer used for?
To determine the concentration of a solution. Since the absorption of that solution is directly proportional to the concentration, the concentration of the solution can be determined.
Does the reaction KCl Na2S result in a colored solution?
No, there is no reaction/
What is thermal reactions?
An Exothermal reaction is a chemical reaction that produces heat making the solution warm or hot. It is the opposite of an Endothemic reaction which makes the solution colder.
Which is more energy given off than taken in A exothermic reaction an endothermic reaction a diluted solution or a concentrated solution?
exothermic
What is solute from solution?
During a chemical reaction, a precipitate is sometimes formed in the solution. The precipitation is the solid that is formed within a solution.
