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They are all basically the same thing. Tris-HCl is just the Tris base converted to a salt with HCl. You can buy either one. The advantage of starting with powdered Tris-HCl is that it is more soluble in water than the base and as a solution has a more neutral pH which is usually the desirable buffer point. Tris base has a pKa of over 8 so using Tris-HCl saves you the trouble of bringing it to a more neutral pH. The one thing to be careful of when making solutions from powder is to be sure to use the correct molecular weight which differs between the two.
To answer your specific question, it doesn't matter which you start with except in the rare cases where the sodium from the NaOH would be an issue. For your situation where the solution is going to be slightly basic, it sounds like you could use either one as the starting reagent. I would go with whatever is already around the lab.
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What is the difference between tris tris cl and tris hcl?
tris tris cl is 6 cl and tris hcl is 3hcl...........
What is Tris HCl?
"Tris" is a chemical compound used as a buffer. The full name is tris(hydroxymethyl)aminomethane. Tris has the ability to absorb counter ions (+H and -OH) so as to help keep the solution that they are in at a stable pH level. When the pH of Tris is set using HCl (hydrochloric acid) the buffer is called Tris HCl.
How do you prepare tris-hcl buffer solution of 0.5M?
6g Tris HCl + 100ml dH2O, pH 6.8
How do you prepare 10mM solution of Tris-HCl?
1.21 g Tris-HCl, QS water to 1L. Scale appropriately.
How do you prepare 0.5M Tris-HCL pH 7.2?
for 1L 121.1 gr tris base 80 ml HcL 920 ml Distilled Water
What is the difference between tris tris cl and tris hcl?
tris tris cl is 6 cl and tris hcl is 3hcl...........
What is the difference between TAE buffer and TE buffer?
The main difference is in composition. In TE common Tris buffer is bring down to pH 8 with HCl and EDTA is involved but in TAE instead of Tris HCl in TE Tris-acetate buffer is used.
What is Tris HCl?
"Tris" is a chemical compound used as a buffer. The full name is tris(hydroxymethyl)aminomethane. Tris has the ability to absorb counter ions (+H and -OH) so as to help keep the solution that they are in at a stable pH level. When the pH of Tris is set using HCl (hydrochloric acid) the buffer is called Tris HCl.
What ia the role of tris-hcl in DNA isolaition of plant?
for any preparation, Tris-HCL does the buffering activity.
How do you prepare 10mM Tris Hcl?
1.21 g Tris-HCl, QS water to 1L. Scale appropriately.
How do you prepare tris-hcl buffer solution of 0.5M?
6g Tris HCl + 100ml dH2O, pH 6.8
How do you prepare 10mM solution of Tris-HCl?
1.21 g Tris-HCl, QS water to 1L. Scale appropriately.
Function of tris HCl in DNA isolation?
Tris is here to maintain pH
How do you prepare 0.5M Tris-HCL pH 7.2?
for 1L 121.1 gr tris base 80 ml HcL 920 ml Distilled Water
What is the buffer capacity of Tris HCl?
7.5-9
How do you prepare 20 mM tris hcl buffer pH 7.4?
to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris
How do you make 62.5 mM Tris-HCl?
mix 6,25 ml of 1 M tris in a total of 100 ml
