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Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering.

In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured.

In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.

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What is turbidimetry?

Turbidimetry is a technique used to measure the amount of light that is scattered by particles in a liquid sample. It is commonly used to determine the concentration of suspended particles in a solution by analyzing changes in the intensity of transmitted light. Turbidimetry is widely applied in various fields such as environmental monitoring, pharmaceuticals, and food and beverage industries.


Why is it important to perform a plate count in conjunction with a turbidimetry procedure?

It is necessary to perform a plate count in conjunction with the turbidimetry procedure because this method will provides us with the exact data of turbidity in our culture for graphing purpose. With the graph we can correlates between corrected O.D. and cell number for our culture.


Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?

Performing a plate count in conjunction with the turbidimetry procedure is essential for validating the accuracy of the turbidity measurements. Turbidimetry provides an estimate of microbial concentration based on light scattering, but it doesn't differentiate between viable and non-viable cells. By comparing the turbidity results with plate counts, which measure only viable cells, researchers can assess the reliability of the turbidity data and ensure appropriate interpretations of microbial populations. This combined approach improves the overall accuracy and reliability of microbial analyses.


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