In the more advanced organic chemistry labs, many introduce fisher esterification by using combinations that yield appealing scents to the students. This specific reaction, when run with small amounts of H2SO4 and one of the reactants in excess will yield an ester with a distinct smell - Bananas. Isoamyl acetate is also the pheromone bees use as a natural alarm, be careful
Similar reactions are run to yield Pineapple oil (ethyl butanoate from ethanol and butanoic acid) and strawberry oil (methyl trans-cinnamate from trans-cinnamic acid with methanol)
banana or pear flavor banana or pear flavor
Pear-like
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
it helps in the removal of proteins from nucleic acid
Did u mean function of chloroform isoamyl alcohol in DNA extraction? Chloroform isoamyl alcohol is a type of detergent. It binds to protein and lipids of cell membrane and dissolve them. By this it will disrupt the bonds that hold the cell membrane together and cause it to breakdown. It then form complexes with these lipids and proteins, causing them to precipitate out of solution. Note that lipids and proteins are non-aqueous compound and DNA/RNA are aqueous compound. The detergent binds to non-aqueous compound.
pekpek
Isoamyl Acetate
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
I am also looking for the same. Do you have some market data for any of the Isoamyl derivative. We can work togehter & share the info.
it helps in the removal of proteins from nucleic acid
Did u mean function of chloroform isoamyl alcohol in DNA extraction? Chloroform isoamyl alcohol is a type of detergent. It binds to protein and lipids of cell membrane and dissolve them. By this it will disrupt the bonds that hold the cell membrane together and cause it to breakdown. It then form complexes with these lipids and proteins, causing them to precipitate out of solution. Note that lipids and proteins are non-aqueous compound and DNA/RNA are aqueous compound. The detergent binds to non-aqueous compound.
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
to separate two layer.DNA is insoluble in alcohol so it will floats up and all other cell components will precipitate at the bottom of the test tube.CIA also helps to isolate the CTAB solution so it will be easy to obtain the DNA.
You can use Isoamyl alcohol instead of octanol. both of them are anti-foaming agents and facilitate separation of phases after centifugation.
[1] The alcohol in alcohol thermometers tends to be pure ethanol, isoamyl acetate, kerosene, or toluene. [2] Ethanol tends to be the most widely used, because of its low cost and its low hazard potential in the event of breakage.
Often these are esters. One such is isoamyl acetate. Please see the link.
=what is the fema ?=
banana oil n. 1. A liquid mixture of amyl acetate and usually nitrocellulose, having a banana-like odor and used as a solvent or flavoring agent. Source: http://www.answers.com/banana+oil?gwp=11&ver=2.3.0.609&method=3
Steam distillation